CDKN2A基因座在膀胱癌中的表达及临床意义

Expression of CDKN2A gene locus in bladder carcinoma and clinical significance

目的:探讨CDKN2A基因座两种编码产物p16INK4A和p14ARF在膀胱癌组织中的表达及其临床意义。方法:采用免疫组织化学技术SP法观察13例正常膀胱组织和52例膀胱尿路上皮癌组织中p16 INK4 A和p14 ARF的表达情况。结果:p16INK4A在正常组织、膀胱癌中阳性表达率分别为84. 62%(11/13)、40. 38%(21/52),两组间差异显著(P <0. 01)。p14ARF在正常膀胱组织、膀胱癌中阳性表达率分别为92. 31%(12/13)、23. 08%(12/52),两组间差异显著(P <0. 01)。p16INK4A和p14ARF的阳性表达率与肿瘤病理分级、临床分期呈负相关(P <0. 05);两者在膀胱癌中的表达没有相关性(P> 0. 05),但他们均与患者的预后正相关(P <0. 05)。结论:p16INK4A和p14ARF在膀胱癌中的表达异常与膀胱癌的发生发展密切相关,但它们所起的生物学作用不尽相同,联合检测两者的表达可能为膀胱癌的早期诊断及预后判断提供帮助。

Objective:To study the expression and implication of p16INK4A and p14ARF in human bladder carcinoma.Methods:Immunohistochemistry method was used to detect p16INK4A and p14ARF expressions in 13 normal bladder tissues and 52 primary bladder carcinoma.Results:The positive expression rate of p16INK4A in normal bladder tissues and primary bladder carcinoma tissues was 84.62%and 40.38%,respectively.There was significant difference between them(P<0.01).The positive expression rate of p14ARF in normal bladder tissues and primary bladder carcinoma tissues was 92.31%and 23.08%,respectively(P<0.01).The positive expression rate of p16INK4A and p14ARF had negative correlation with clincopathological stages and pathlolgyical grades(P<0.05).The expression of p16INK4A and p14ARF in bladder carcinoma had not a positive correlation(P>0.05).But there were relation between p16INK4A,p14ARF and prognosis(P<0.05).Conclusion:Expression of p16INK4A and p14ARF in bladder carcinoma play an important role in suppression of invasive growth and metastasis of tumor,which might contribute to various biological behaviors.United detection of p16INK4A and p14ARF might be acted as an marker of bladder carcinoma diagnosis and prognosis.