五味子胚状体途径组培苗的壮苗及移栽条件研究

Study on Seedling and Transplanting Conditions of Embryoid Pathway Tissue Culture of Schisandra Chinensis

本研究以五味子优系"早红"胚状体组培苗为材料,研究不同壮苗及移栽条件对其生长量以及移栽成活率的影响。结果表明,五味子组培苗接种于1/2MS培养基,在白色光源下每天光照12h其长势较好;移栽于基质为草炭土∶蛭石=3∶1、环境湿度80%～90%、保湿时间12d条件下组培苗移栽成活率较高,可达100%。

This study,with the tissue culture plants of Schisandra chinensis by means of embryoid as the experimental material.The effects of different seedlings and transplanting conditions on the growth situation and survival rate of the S. chinensis after transplantation were studied.The results showed that the best conditions of strengthening plantlet were 1/2 MS medium and under the white light condition of12 hours one day;the best conditions of transplanting werethe straw charcoal∶vermiculite = 3∶1 media with humidity at 80%-90%condition of 12 days,to which the survival rates reached 100.0%.