聚类分析法研究不同产地花生壳高效液相色谱指纹图谱

HPLC fingerprint of peanut hulls from different habitats by cluster analysis

对8个产地的花生壳样品进行了HPLC指纹图谱研究,优化了样品提取条件和色谱测定条件,并进行了聚类分析。优化的色谱条件为:Wonda Cract ODS-2 C_(18)(4.6 mm×150 mm,5μm)色谱柱,以乙腈-0.5%磷酸溶液为流动相进行梯度洗脱,流速1 mL·min^(-1),柱温35℃,波长290 nm。建立8个产地花生壳样品的HPLC指纹图谱,标示出14个稳定的共有峰,共有模式的相似度除新昌小京生外,皆大于0.9。对共有峰平均相对峰面积的聚类分析表明,8种花生壳可分为两大类。

The HPLC fingerprints of peanut shell samples from 8 habitats were studied and cluster analysis was carried out.The conditions of sample extraction and chromatographic were optimized.The optimized chromatographic conditions were WondaCract ODS-2 C 18(4.6 mm×150 mm,5μm)column and gradient elution with acetonitrile-0.5%phosphate solution as mobile phase.The flow rate was 1 mL·min-1,the column temperature was 35℃,and the wavelength was 290 nm.The HPLC fingerprints of peanut shell samples from 8 regions were established,indicating 14 stable common peaks.The similarity of common patterns was larger than 0.9 except Xinchang Xiaojingsheng.The cluster analysis of the average peak area of the common peaks showed that the 8 peanut shells could be divided into two categories.