肺结核患者外周血MAIT细胞功能及血清IL-22水平分析

The role Function of peripheral blood mucosal-associated invariant T cells and serum IL-22 in level in patients with active pulmonary tuberculosis

目的观察肺结核(TB)患者外周血MAIT细胞功能及血清白细胞介素22(IL-22)水平,分析其临床意义。方法收集50例TB患者和30例健康志愿者为研究对象。流式细胞术检测外周血MAIT细胞水平;酶联免疫吸附试验(ELISA)检测血清IL-22水平;磁珠分选CD8+T细胞,体外结核菌抗原Ag85B刺激检测MAIT细胞产生Perforin和IL-22的能力。结果 TB患者外周血MAIT细胞和血清IL-22水平均明显低于健康志愿者(P<0.05);痰涂片检查结核菌阳性患者外周血MAIT细胞和血清IL-22水平均明显低于阴性患者(P<0.05);常规化疗药物治疗后外周血MAIT细胞和血清IL-22水平均明显增高(P<0.05);TB患者外周血MAIT细胞和血清IL-22水平呈正相关(P<0.05);Ag85B干预后,TB患者和健康志愿者外周血MAIT细胞产生Perforin的能力均明显降低(P<0.05),而产生IL-22的能力与对照组比较无明显差异(P>0.05)。结论MAIT细胞与IL-22可能在抗结核免疫过程中发挥重要作用。

Objective To study observe the role function of peripheral blood mucosal-associated invariant T(MAIT)cellsMAIT cells and serum IL-22 level in peripheral blood ofthe patients with pulmonary tuberculosis(TB)and to analysis analyze its their clinical significance.Methods 50 Fiftycases patients with TB and 30 cases healthy volunteers(HV)were collected as the study subjects.The counts level of peripheral blood MAIT in cells peripheral blood wereas detected by flow cytometry.The levels of serum IL-22 level in serum werewas detected by ELISA.CD8+T cells were sorted by magnetic beads.The production ability of MAIT cells for producing Perforin and IL-22 by MAIT cells werewas examined by flow cytometry after in vitro Mycobacterium tuberculosis antigen Ag85B stimulatedstimulation.Results The counts levels of peripheral blood MAIT and serum levels of IL-22 in peripheral blood ofthe patients with TB were significantly lower than those of in healthy volunteers(P<0.05).The levels of peripheral blood MAIT cells and serum IL-22 in the patients with Mycobacterium tuberculosis positive by sputum smear were significantly lower than those in the patients with Mycobacterium tuberculosis negative(P<0.05).The levels of peripheral blood MAIT cell countss and serum IL-22 after routine chemotherapy and were significantly increased(P<0.05);the level of peripheral blood MAIT cells was positively correlated with serum IL-22 levels level(P<0.05);after Ag85B intervention,the ability of peripheral blood MAIT cells for producing Perporin in the TB patients and healthy volunteers was significantly decreased(P<0.05),while the ability for producing IL-22 had no obvious difference compared with the control group(P>0.05)in sputum smear-positive TB patients were significantly lower than those of sputum smear-negative TB patients(P<0.05).There was a positive correlation between MAIT cell counts and serum IL-22 levels in patients with TB(P<0.05).However,there was no significant difference in IL-22 production between the two groups(P>0.05).Conclusion MAIT cells and IL-22 may play an important role in during anti-TB immunity process.