香鳞毛蕨有效部位体外抗犬小孢子菌作用及其机制研究

Study on antifungal effect and mechanism of Dryopteris fragrans(L.) Schott effective parts on Microsporum canis in vitro

目的探讨香鳞毛蕨有效部位(DF)体外抗犬小孢子菌(Microsporum canis)的作用及机制。方法采用微量稀释法测定DF对M.canis的最低抑菌浓度(MIC)以及最低杀菌浓度(MFC),从生物量的生成、山梨糖醇保护试验、麦角甾醇结合试验、细胞泄漏以及对麦角甾醇含量的影响等角度,探究DF的抗菌机制。结果 DF对M.canis的MIC及MFC几何均值的范围分别为0.298～0.596 mg/mL和0.334～0.668mg/mL。与生长对照组相比,DF高剂量组、阳性药物组均能显著性降低M.canis的生物量和麦角甾醇的含量(P<0.01),和明显增加胞内物质的释放(P<0.01);无论是否加入外源性山梨糖醇和麦角甾醇,DF对M.canis的MIC值均无变化。结论 DF对M.canis具有良好的体外抗菌效果,其机制可能是通过影响麦角甾醇的合成,或破坏细胞的完整性而实现。

Objective To study the antifungal effect and mechanism of Dryopteris fragrans(L.)Schott effective parts(DF)on Microsporum canis(M.canis)in vitro.Methods Microdilution method was used to determine the Minimal Inhibitory Concentration(MIC)and the Minimal Fungicide Concentration(MFC)of DF on M.canis.Biomass dry weight assay,sorbitol protect assay,ergosterol binding assay,cell leakage assay,ergosterol quantitation assay were involved to study the mechanism of DF against M.canis.Results The MIC and MFC ranges of geometric mean about DF on M.canis were0.298~0.596mg/mL and0.334~0.668mg/mL respectively.Compared with control group,high dose group of DF and reference medicine groups could decrease dry weight of biomass and quantitation of ergosterol significantly(P<0.01),and could increase the intracellular material obviously(P<0.01).The MIC values of DF with and without sorbitol and ergosterol were identical.Conclusion DF has good antifungal effect on M.canis,the mechanism of action may be related to destruction of cell integrity and effect on ergosterol biosynthesis.