表皮细胞生长因子对小鼠非酒精性脂肪肝炎TNF-α、CHOP的影响

Effect of epidermal growth factor on the expression of TNF-α and CHOP in non-alcoholic steatohepatitis

目的探讨表皮生长因子(EGF)对小鼠细胞非酒精性脂肪性肝炎(NASH)模型炎症因子及内质网应激的影响。方法用蛋氨酸与胆碱缺乏(MCD)的培养基诱导AML12小鼠正常肝细胞株建立NASH模型,分别设空白对照组、NASH造模组、EGF组,Western blot检测各组肿瘤坏死因子α(TNF-α)、C/EBP同源蛋白(CHOP)蛋白表达,油红O染色检测细胞内脂质沉积程度,实时定量荧光PCR(real-time PCR)检测TNF-α、CHOP的mRNA转录水平。结果 EGF组TNF-α、CHOP的蛋白及mRNA相对表达量与NASH组比较显著下降(P<0.05),脂质沉积变化不明显。结论 EGF能明显下调NASH中TNF-α、CHOP的表达水平,但对脂质沉积无明显改变。

Objective To explore the therapeutic effect of epidermal growth factor(EGF)to inflammatory factor and endoplasmic reticulum stress(ERS)on mouse non-alcoholic steatohepatitis(NASH).Methods NASH model was established by using methionine-choline-deficient(MCD)medium to induce normal liver cell line of AML12mice.Tumor necrosis factor-α(TNF-α)and C/EBP homologous protein(CHOP)were detected by western blot.Oil red O staining was used to detect the intracellular lipid deposition.Real-time quantitative fluorescent PCR(Real-time PCR)was used to detect TNF-αand CHOP mRNA expression.Results The levels of TNF-αand CHOP in the drug-treated group were significantly lower than those in the control group(P<0.05),and the lipid deposition was not obvious different between two groups.Conclusion EGF can significantly down-regulate the expression of TNF-αand CHOP in NASH,but there is no significant change in lipid deposition.