甲醛暴露调节海马GR抑制雌性小鼠的学习记忆

Formaldehyde Exposureinhibits Learning and Memory Through GR in Hippocampus in Female Mice

探讨甲醛对雌性小鼠学习记忆的影响及其机理。以C57BL/6J小鼠为研究对象,分为对照组、0.5 mg/m^3组和2.5 mg/m^3组。小鼠连续吸入甲醛气体14 d,每天2 h,利用水迷宫实验评估小鼠的空间学习记忆能力,免疫组织化学法和westornblot方法检测海马GR的表达。结果表明,在训练期,与对照组相比,吸入2 mg/m^3甲醛浓度组的小鼠逃避潜伏期显著增加,而且在检测期,2.5mg/m^3组的雌性小鼠到达平台的时间明显延长,穿越平台的次数明显减少; 2 mg/m^3组小鼠海马CA1和CA2/3区的GR阳性神经元的数目比对照组显著降低; 2.5 mg/m^3甲醛浓度组的小鼠海马GR蛋白的表达显著降低。但这些结果在0.5 mg/m^3组雌性小鼠都没有显著性的改变。提示甲醛对小鼠学习记忆的影响是通过对海马GR的降低所引起的。

To investigate the effect and mechanism of formaldehyde on learning and memory in female mice.C57BL/6J mice were divided in to three groups:contorl,0.4 mg/m^3 and 2 mg/m^3·Mice inhaled formaldehyde air for 14 days,and 2 h one day.The spatial learning and memory ability of mice was evaluated using water maze experiment.Immunohistochemical method and westorn blot method were used to detect the expression of GR in hippocampus.The results of water maze test showed that the escape latency of mice exposed to 2 mg/m^3 formaldehyde was significantly increased during the training period,and the time of reaching the platform of female mice in the 2.5 mg/m^3 group was significantly longer than that in the control group.The frequency of crossing the platform was obviously reduced.Compared with the control group,the number of GR positive neurons decreased significantlyin the CA1 and CA2/3 regions in hippocampus at 2 mg/m^3 formaldehyde concentration group,and compared with the control,the expression of GR protein was significantly decreased in the hippocampus at 2.5 mg/m^3 group.However,these results had no significant change in the 0.5 mg/m^3 group.It is suggest that the effect of formaldehyde on learning and memory in mice is caused by the decrease of hippocampal GR.