帕瑞昔布对大鼠肺缺血再灌注损伤及水通道蛋白1的影响

Effects of Paroxib on Lung Ischemia-reperfusion Injury and Aquaporin-1 in Rats

目的探讨帕瑞昔布在大鼠肺缺血再灌注(LIR)损伤的作用及对肺内水通道蛋白-1(AQP-1)的影响。方法24只雄性成年SD大鼠随机分为3组:假手术组(C组)、缺血再灌注组(I组)和帕瑞昔布组(P组)。建立单侧LIR模型,检测肺湿/干重比值(W/D),观察肺组织病理变化,检测肺组织内TNF-α、IL-6含量,AQP-1表达情况。结果 LIR后,肺组织内TNF-α、IL-6明显增多(P<0. 05),肺湿/干重比值也增加(P<0. 05);与I组相比,以上指标在P组中均降低(P<0. 05)。肺组织形态学检测显示,LIR造成肺组织损伤严重,但P组较I组明显减轻。与C组相比,缺血再灌注明显抑制肺组织内AQP-1表达(P<0. 05);但P组表达水平较I组高(P<0. 05)。结论帕瑞昔布可抑制肺缺血再灌注引起的炎症反应,增加AQP-1表达,从而减轻损伤。

Objective To investigate the effect of paroxib on lung ischemia reperfusion(LIR)injury and its effect on intrapulmonary aquaporin-1(AQP-1)in rats.Methods Twenty-four adult male SD rats were randomly divided into three groups:sham operation group(group C),ischemia reperfusion group(group I)and paroxib group(group P).A unilateral LIR model was established to detect the lung wet/dry weight ratio(W/D),to observe the pathological changes of lung tissue,and to detect the content of TNF-α,IL-6and the expression of AQP-1in lung tissue.Results After LIR,TNF-αand IL-6in lung tissue increased significantly(P<0.05),and the lung wet/dry weight ratio also increased(P<0.05);Compared with group I,the above indexes were decreased in group P(P<0.05).Lung histomorphologic examination showed that LIR caused severe lung injury,but group P was significantly less than group I.Compared with group C,ischemia reperfusion significantly inhibited the expression of AQP-1in lung tissue(P<0.05);But the expression level of AQP-1in group P was higher than that in group I(P<0.05).Conclusion Parecoxib can inhibit the inflammatory response and increases the expression of AQP-1,thereby reducing the damage induced by lung ischemia-reperfusion.