Pim-1在原发性肝细胞癌组织中的表达情况及其临床意义

Expression of Pim-1 in primary hepatocellular carcinoma and its clinical significance

目的明确Pim-1在原发性肝细胞癌(PHC)组织中的表达及其临床意义。方法采用免疫组织化学染色(IHC)方法检测122例PHC、相应的癌旁及85例正常肝组织中Pim-1蛋白的表达情况。回顾性分析Pim-1蛋白表达水平与临床病理指标的关系。Kaplan-Meier法计算不同Pim-1表达水平对PHC患者生存情况的影响。采用Cox比例风险回归模型分析影响PHC患者生存期的危险因素。结果 Pim-1蛋白在PHC组织中的总阳性率为88. 5%,显著高于癌旁组织(73. 0%)和正常肝组织(69. 4%)(P <0. 05)。单因素分析表明,Pim-1蛋白高表达患者的术前肝功能较差、肿瘤数目较多、肿瘤直径较大、淋巴结转移发生率较高且TNM分期较高(P <0. 05)。生存分析提示与Pim-1高表达组患者相比,低表达组患者的生存期延长(P <0. 05)。多因素分析表明,高表达Pim-1蛋白是影响PHC患者生存时间的独立危险因素(P <0. 001)。结论 Pim-1在PHC组织中的表达水平显著升高,与PHC的临床进展和患者生存时间有一定的关联性,其过表达提示PHC患者的预后较差。

Objective The aim of this study was to determine the expression of Pim-1 in primary hepatocellular carcinoma(PHC)and its clinical significance.Methods Immunohistochemical staining(IHC)was used to detect the expression of Pim-1 in 122 cases of PHC tissues,corresponding paracancer tissues,and 85 normal liver tissues.The relationship between the expression of Pim-1 protein and clinicopathological parameters was analyzed retrospectively.K-M method was used to analyze the effect of different Pim-1 expression on the survival time of PHC patients.Cox proportional hazard regression models were used to analyze risk factors affecting survival time in patients with PHC.Results The total positive expression rate of Pim-1 protein in PHC tissues was 88.5%,which was significantly higher than that in adjacent tissues and normal liver tissues(P<0.05).Univariate statistical analysis showed that patients with high expression of Pim-1 protein had poor preoperative liver function,more tumors,larger tumor diameter,high incidence of lymph node metastasis and high TNM stage(P<0.05).Survival analysis suggested that the survival time of patients in the low expression group was significantly longer than that in the high expression of Pim-1 group(P<0.05).Multivariate statistical analysis showed that high expression of Pim-1 protein was an independent risk factor for survival time in patients with PHC(P<0.001).Conclusion The expression level of Pim-1 in PHC tissues is significantly increased,which is related to the clinical progress of PHC and the survival time of patients.Pim-1 overexpression indicates the poor prognosis of PHC patients.