甘蓝晚抽薹基因BoFLC3克隆、序列分析和亚细胞定位

Cloning,sequence analysis and subcellular localization of Brassica oleraceaBoFLC3 gene

参照甘蓝FLC3基因(GenBank登录号AAP31677)碱基序列设计引物,扩增出甘蓝晚抽薹BoFLC3基因,序列分析结果表明,该基因编码的蛋白质有197个氨基酸,分子量20 990,等电点9. 45。荧光定量PCR分析结果表明:抽薹后BoFLC3基因在甘蓝不同部位表达存在差异,表达量从高到低依次为:叶、茎、花蕾、花瓣和根。亚细胞定位结果表明:BoFLC3蛋白定位在细胞核。

According to the sequence of FLC3 gene(GenBank accession number AAP31677),primers were designed to amplify the BoFLC3 gene of Brassica oleracea L.Sequence analysis showed that the gene encoded a protein containing 197 amino acids,the molecular was 20 990,and the isoelectric point was 9.45.Quantitative real-time PCR analysis results showed that the expression of BoFLC3 gene in different parts of cabbage after bolting was different,and the order of expression from high to low was leaves,stems,buds,flowers and roots.Subcellular localization results showed that the BoFLC3 protein was located in the nucleus.