Aβ_(1-42)寡聚体对小鼠神经胶质细胞D1a IL-1β表达水平的影响研究

The effect of Aβ_(1-42) oligomer on IL-1β expression in rat glial cell D1a

目的探讨β-淀粉样蛋白1～42寡聚体(Aβ1-42)寡聚体对小鼠神经胶质细胞D1a白细胞介素(IL)-1β表达水平的影响。方法采用Aβ1-42寡聚体和二甲基亚砜(DMSO)小鼠神经胶质细胞D1a,采用蛋白免疫印迹法(Westernblot)、实时荧光定量逆转录聚合酶链反应(qRT-PCR)、免疫荧光染色技术检测并比较D1a细胞IL-1β蛋白和m RNA表达水平的差异。结果与DMSO处理相比,Aβ1-42寡聚体处理可明显上调D1a细胞IL-1β蛋白和mRNA表达水平(P<0.05)。结论 Aβ1-42寡聚体可能通过活化神经胶质细胞上调IL-1β的表达。

objective To explore the effect of amyloidβ-protein 1-42 oligomers(Aβ1-42 oligomers)on interleukin-1β(IL-1β)expression in rat glial cell D1a.Methods D1a cells were treated with Aβ1-42 oligomer and dimethyl sulfoxide respectively.The expression of IL-1βprotein and mRNA were detected by using Western blot,quantitative real-time polymerase chain reaction and immunofluorescence.Results The expression levels of IL-1βprotein and mRNA in D1a cells treated with Aβ1-42 oligomers were significantly higher than those treated with DMSO(P<0.05).Conclusion Aβ1-42 oligomers could activate the glial cells and up-regulate the expression of IL-1β.