2013-2016年云南省江城县牛羊蓝舌病血清流行病学调查

Serological Investigation of Bluetongue in Cattles and Goats in Jiangcheng County of Yunnan Province during 2013 to 2016

为了解近年来云南省江城县蓝舌病病毒(BTV)的感染和流行情况,从2013年5月至2016年4月连续3年在江城县设立监控动物群,每年选择BTV病原及抗体检测阴性牛10头、羊5只作为哨兵动物,用于BTV抗体监测和病毒分离。应用BTV C-ELISA抗体检测试剂盒,对哨兵动物进行血清抗体检测,应用鸡胚、C6/36细胞和BHK-21细胞进行病毒分离。结果显示:连续3年的哨兵动物BTV抗体阳性率分别为40.35%、44.08%、50.65%; 3年内共分离到45株BTV,包括1～5型、15～16型、21型和24型共9种血清型。结果表明,江城县蓝舌病流行有逐年加重趋势,且呈现多种血清型毒株交叉感染状况,亟需加大BTV监测力度,及时发现毒株变化。

In order to recognize the infection and prevalence situation of bluetongue virus(BTV)in Jiangcheng County of Yunnan Province in recent years,during May2013to April2016,a group of animals were used to monitor the disease.Specifically,10cattle and5goats without BTV antigen and antibody were selected as sentinel animals to monitor and isolate BTV every year,The BTV C-ELISA kits were used to detect serum antibodies in sentinel animals,and chicken embryos,C6/36cells and BHK-21cells were used for virus isolation.Results showed that,in the three consecutive years,the positive rates of antibody against BTV in sentinel animals were40.35%,44.08%and50.65%,respectively.A total of45strains of BTV were isolated,including9serotypes of BTV-1,BTV-2,BTV-3,BTV-4,BTV-5,BTV-15,BTV-16,BTV-21and BTV-24.As a conclusion,BTV infection in Jiangcheng County was serious year by year,and cross-infection of multiple serotypes existed,therefore,it was urgent to strengthen the monitoring of local bluetongue virus circulation.