摘要
外泌体是由细胞分泌的膜性囊泡,是细胞间通讯的重要介质,参与到细胞间生物信号的传递。目前有5种公认且较为常用的提取外泌体的方法。外泌体在临床疾病研究中突破性的进展主要体现在外泌体参与肿瘤疾病的进展、外泌体中的核酸或蛋白可以作为疾病的分子标志物、外泌体可以作为药物的载体进行靶向治疗等方面。外泌体在疾病中行使的功能使其极具向临床应用的优势。本文主要从外泌体的发现、提取方法优缺点及其在临床疾病功能的研究进展这三个方面加以阐述。
Exosomes are membranous vesicles secreted by cells,which are important mediators for cell communication and participate in the transmission of biological signals between cells.There are five recognized and more commonly used methods of extracting exosomes.The breakthrough of exosomes in clinical research mainly reflects on that exosomes are involved in the development of tumor diseases,nucleic acids or proteins in exosomes can be used as molecular markers of diseases,and exosomes can be used as drug carriers for targeted therapy.The function of exosomes in diseases gives them an advantage in clinical application.This review focuses on the discovery of exosomes,the advantages and disadvantages of exosomes extraction methods and the research progress of exosomes function in clinical diseases.
作者
魏晓晶
胡晓
WEI Xiaojing;HU Xiao(State Key Laboratory of Experimental Hematology, Hematology and Blood Diseases Hospital & Institute of Hematology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China;Department of Stem Cell and Regenerative Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China)
出处
《中国医药导报》
CAS
2018年第34期45-48,共4页
China Medical Herald
基金
国家重大科学研究计划课题(2012CB966504)
科技部十二五863计划生物和医药技术领域干细胞治疗技术临床转化及应用研究主题项目(2011AA020118)
天津市应用基础及前沿技术研究计划面上项目(11JCYBJC27400)
关键词
外泌体
提取方法
外泌体与临床疾病
Exosome
Extraction methods
Exosomes and clinical diseases