黑青稞根际促生菌筛选及其对种子萌发的影响

Selection of Rhizotrophic Bacteria from Rhizosphere and Its Effect on Seed Germination of Black Barley

对西藏山南隆子县黑青稞根际促生菌分别进行筛选溶磷、固氮能力的菌株,测定其溶磷、固氮能力,并评价其对黑青稞种子萌发的促生作用,以期为青稞微生物肥料的研制提供优良的菌株资源。结果表明,通过透明圈法初筛及定量测定复筛出固氮细菌、解无机磷细菌、解有机磷细菌各4株。分别用筛选出的菌株的菌液对小麦种子进行浸种处理,HQ 3-3、HQ 7-4和SR 15-2菌株对青稞种子发芽促进作用显著,与ck相比,发芽率分别增长了36.05%、29.07%和29.07%;菌株SR 19-1和LQ 3-3对青稞种子芽生长有显著的促进作用,分别较对照增加30.02%和41.55%。HQ 3-5和HQ 7-4菌株对青稞幼苗地下部分生长有显著的促进作用,分别较对照显著增加89.31%和86.30%。综上,不同菌株对青稞种子及幼苗的促生作用不同,但同时也筛选出了青稞种子萌发及幼苗生长都有促进作用的菌株固氮菌HQ 7-4、解有机磷HQ 36-2、解无机磷细菌SR 6-2、SR 19-1,为青稞微生物菌肥的研制提供了功能菌株。

The material is black highland barley in longzi county,Tibet Shannan.Their bacteria were screened strains of dissolved phosphorus,nitrogen fixation ability.It was measured its soluble phosphorus and nitrogen fixation ability,and evaluated its role in the growth of black highland barley seed germination,In order to provide excellent strain resources for the development of highland barley microbial fertilizer.The results showed that 4 strains of nitrogen fixing bacteria,4 phosphate solubilizing bacteria and 4 organic phosphate solubilizing bacteria were screened out by the transparent circle method.The HQ 3-3,HQ 7-4 and SR 15-2 were significant promoted to seed germination of highland barley,respectively.The germination rate of highland barley was increased by 36.05%,29.07%and 29.07%,respectively.SR 19-1 and LQ 3-3 had significant promotion effect on the growth of the seed buds of highland barley,which increased by 30.02%or 41.55%respectively compare to control.HQ 3-5 and HQ 7-4 strains significantly promoted the growth of the underground parts of highland barley seedlings,which increased by 89.31%or 86.30%respectively compare to control.In conclusion,Different strains have different growth-promoting effects on either the germination or growth to highland barley.While,Both the germination promotion and growth promotion were screened in the strains of nitrogen fixing bacteria HQ 7-4,organic phosphorus HQ 36-2,inorganic phosphorus bacteria SR 6-2 and SR 19-1.