茶树CsMYB基因启动子的克隆与功能分析

Cloning and Functional Analysis of the CsMYB Promoter In Tea Plant(Camellia sinensis L.)

以茶树新品系"1005"嫩芽为材料,克隆得到CsMYB基因gDNA全长序列1 828 bp,通过染色体步移技术,分离得到CsMYB基因启动子片段1 038 bp,命名为proMYB。生物信息学分析表明,CsMYBgDNA由2个内含子和3个外显子组成,该启动子含有与提高基因转录水平相关的5′UTR Py-rich stretch顺式作用元件、启动子核心元件TATA-box和CAAT-box,还存在有激素响应元件、光响应元件、逆境应答元件以及大量功能未知或功能特异的顺式作用元件,说明proMYB具有诱导型启动子特性,CsMYB基因在茶树植物体中可能参与多种非生物胁迫应答和激素信号传导;通过烟草的瞬时表达结果表明,该启动子能驱动下游基因表达,具有启动子活性。

The 1 828 bp full-length gDNA of CsMYB and a 1 038 bp 5′-flanking sequence of CsMYB(named proMYB)were cloned from the shoots of tea cultivar‘1005’by gDNA cloning and genome-walking method.Bioinformatics analysis showed that the gDNA of CsMYB contained 2 introns and 3 extrons.The cis-acting element analysis showed that the proMYB promoter contained 5′-UTR Py-rich stretch motifs,TATA-box,CAAT-box core elements putative elements responding to hormone,light,stresses and some cis-elements with unknown or specific function,suggesting the inducible character of proMYB.It was also implied that CsMYB might be involved in abiotic stress responses and hormonal signal transduction in tea plant.The transient expression in transformed tobacco showed that the proMYB could drive the expressions of downstream genes.