大动脉粥样硬化型缺血性脑卒中患者SLCO1B1基因多态性分布——185例分析

Distribution of SLCO1B1 Gene Polymorphism in 185 Patients with Large Artery Atherosclerosis-Subtype Ischemic Stroke

目的:分析大动脉粥样硬化型缺血性脑卒中(LAA-IS)患者185例中SLCO1B1基因388A>G和521T>C多态性位点的分布。方法:LAA-IS患者185例纳入研究,通过聚合酶链反应-荧光探针方法对其外周血样本中SLCO1B1基因的388G>A和521T>C位点进行检测并统计分析其基因分型分布。结果:根据SLCO1B1基因多态性分型,185例LAA-IS患者中SLCO1B1基因388A>G位点纯合野生型占8.6%,杂合突变型占40.5%,纯合突变型占50.8%;521T>C位点纯合野生型占77.8%,杂合突变型占21.0%,纯合突变型占1.1%。结论:本组185例LAA-IS患者中分布有较多的突变型SLCO1B1基因。

Objective:To analyze the distribution of the SLCO1B1 gene 388A>G and 521T>C polymorphic loci in 185 patients with large artery atherosclerosis-subtype ischemic stroke(LAA-IS).Methods:This study recruited 185 LAA-IS patients as research subjects.The 388G>A and 521T>C loci of the SLCO1B1 gene in the peripheral blood samples were detected by the polymerase chain reaction fluorescence probe method,and the distribution of their genotyping was statistically analyzed.Results:According to the polymorphism of the SLCO1B1 gene in the 185 LAA-IS patients,at the 388A>G site,homozygous wild type accounted for 8.6%,heterozygous mutant type accounted for 40.5%,and homozygous mutant type accounted for 50.8%;at the 521T>C site,homozygous wild type accounted for 77.8%,heterozygous mutant type accounted for 21.0%,and homozygous mutant type accounted for 1.1%.Conclusion:Within the 185 cases in this study,there exists a large distribution of mutant genotypes of the SLCO1B1 gene.