有性杂交结合胚挽救在无核葡萄育种上的应用

Application of Sexual Hybridization Combined with Embryo Rescue in Seedless Grape Breeding

大粒、无核是鲜食葡萄育种的重要目标,文章主要介绍了利用胚挽救进行无核葡萄育种的流程及胚培养技术要点,以无核葡萄作为母本,优质大粒的欧美种或欧亚种作父本进行杂交育种,在胚没有完全败育前采集田间幼果,无菌条件下利用培养基促进胚的萌发,进而培养成苗,获得无核葡萄种苗。

Large grains and seedless grapes are important objectives of fresh grape breeding.This paper mainly introduces the process of seedless grape breeding by embryo rescue and the key points of embryo culture technology.The seedless grape was used as female parent,the European and American or Eurasian varieties with high quality and large grains as male parent for cross breeding.The young fruits were collected before the embryo was completely aborted.The germination of embryo was promoted by using culture medium under aseptic condition,and then seedlings were cultured,to obtain seedings of seedless grape.