3种品系小鼠iNKT细胞组织特异性分布

Tissue-specific distribution of iNKT cells in three strains of mice

目的比较3种品系小鼠外周血、胸腺、脾脏和肝脏中iNKT细胞频率及胸腺、脾脏、肝脏iNKT细胞中iNKT1、iNKT2亚群比例的差异。方法分别以DBA/1、NOD/Lt J及C57BL/6J 3种品系小鼠为研究对象,分离健康小鼠外周血、胸腺、脾脏和肝脏的淋巴细胞,采用FCM检测各组织器官中TCRβ和CD1d四聚体双阳性的iNKT细胞频率,并根据核内PLZF和T-bet的表达水平确定iNKT1和iNKT2亚群。结果 (1) 3种品系小鼠外周血中iNKT细胞频率最低,肝脏中iNKT细胞频率最高,脾脏和胸腺iNKT细胞频率介于两者之间。其中,C57BL/6J小鼠胸腺iNKT细胞频率显著低于DBA/1及NOD/Lt J小鼠,肝脏iNKT细胞频率显著高于其他2品系小鼠; NOD/Lt J小鼠脾脏、肝脏iNKT细胞频率显著低于C57BL/6J及DBA/1小鼠。(2) 3种品系小鼠胸腺iNKT细胞中iNKT2亚群占比较高,脾脏、肝脏中iNKT细胞以iNKT1亚群为主。其中,C57BL/6J小鼠胸腺iNKT2亚群比例最低; NOD/Lt J小鼠胸腺iNKT2亚群比例最高,脾脏、肝脏iNKT1亚群比例较高。结论 DBA/1、NOD/Lt J及C57BL/6J 3种品系小鼠,同一品系小鼠不同组织器官iNKT细胞频率及亚群比例,不同品系小鼠同一组织器官iNKT细胞频率及亚群比例存在很大差异。

Objective To compare the rates of iNKT cells in peripheral blood,thymus,spleen,liver and the proportion of iNKT1,iNKT2in thymus,spleen,liver respectively of three strains mice:DBA/1,NOD/LtJ and C57BL/6J.Methods Isolation of lymphocytes from peripheral blood,thymus,spleen and liver of mice.TCRβand CD1d tetramers were detected by using FCM to identify the rates of iNKT cells,endonuclear PLZF and T-bet were detected to confirm iNKT1and iNKT2subgroup in lymphocytes of these tissues.Results(1)The rates of iNKT cells was the lowest in the peripheral blood and the highest in the liver in all the three strains of mice.Compared with DBA/1and NOD/LtJ mice,the rates of iNKT cells in C57BL/6J mice was lower in thymus,but was higher in liver.Compared with C57BL/6J and DBA/1mice,the rates of iNKT cells in NOD/LtJ mice was lower in spleen and liver.(2)In all the three strains of mice,the proportion of iNKT2was relatively high in thymus,iNKT1was relatively high in spleen and liver.Among them,C57BL/6J mice was the lowest proportion of iNKT2in thymus,and NOD/LtJ mice had the higher proportion of iNKT1in spleen and liver.Conclusion In the DBA/1,NOD/LtJ and C57BL/6J mice,the rates and subpopulation ratio of iNKT cells were significantly different in different tissues of the same strain mice and in different strains mice of the same tissue.