响应面法优化豆渣纤维酶解多糖制备工艺及其抗氧化活性研究

Optimizing Enzymatic Preparation for Polysaccharides by Response Surface to Soybean Residue and Its Antioxidant Activities

豆渣纤维是大豆加工副产物豆渣的主要成分,以豆渣纤维为底物,通过酶法水解制备水溶性多糖(Polysaccharides from soybean residue,SRP),响应面法优化制备工艺,并对水解所得SRP抗氧化活性进行评价。通过研究酶用量、pH和温度对SRP制备的影响,在单因素试验基础上进行响应面优化最佳制备工艺条件。SRP最佳制备工艺参数为:酶用量60 U/g、pH 5.10、温度50℃,在此条件下,酶解1 h所得SRP得率为45.71%。在此条件下,水解不同时段得到多糖产物抗氧化活性进行研究,确定最佳抗氧化SRP片段。结果表明,最佳制备工艺条件下,降解时间8 h所得SRP组分具有最佳抗氧化活性。

Soybean crude fiber(SCF)is the major component of soy dregs,a byproduct from soybean production.Water-soluble polysaccharide(SRP)is produced by enzymatic hydrolysis of SCF.This study used SCF as the substrate,prepared SRP by enzyme methodology,and optimized the preparation by response surface to assess the antioxidant activities.The effects of enzymes amount,pH and temperature on SRP preparation were investigated.The optimum hydrolysis conditions for SRP were60U/g,temperature of50℃and pH of5.10.Under these conditions,1hour degradation produced45.71%SRP.The optimum antioxidant SRP fractions were obtained.The results showed that SRP has significant total reducing capacity and high scavenging activity on hydroxide radical(·OH).SRP fractions obtained from8h hydrolysis showed the best antioxidant activities.