深Ⅱ度烧伤皮肤miRNA差异表达研究

Study on differential expression of the mi-RNA in the deep second degree burns

目的:研究深Ⅱ度烧伤皮肤与正常皮肤组织中miRNA差异表达谱,探讨差异表达miRNAs在创面愈合中的作用。方法:利用miRNA基因芯片检测技术筛选出深Ⅱ度烧伤皮肤与正常皮肤组织差异表达的miRNA,运用实时定量RT-PCR验证部分miRNA表达情况。通过基因数据库TargetScan、miRanda和mirbase进行靶基因预测。对预测的靶基因利用GO-Analysis进行靶向性功能分析及Pathway进行信号通路分析。结果:深Ⅱ度组与正常组相比,上调的有41个,下调的有124个;其中miR-210-3P,miR-99a-5P,miR-99b-3P,miR-99b-5P,miR-143-5P,miR-29c-5P,miR-15b-5P,miR-323a-3P,miR-424-5P,miR-125b-1-3P,miR-125a-3P,miRNA-184差异表达显著,且参与创面炎症反应、免疫应答及愈合过程。结论:在基因水平上确定差异表达的miRNAs及其对应靶基因在烧伤创面愈合中的调控机制,为烧伤创面精准治疗提供靶点。

AIM:To study the differential expression profile of the miRNA in deep second-degree burns and normal skin tissue,and to investigate the effects of differential expression of the miRNA on wound healing.METHODS:The differential expression of miRNA in deep second-degree burns and normal skin tissue were screened by gene chip detection technology,and the expression of some miRNA was verified by RT-PCR.Target gene prediction was carried out through gene databases TargetScan,miRanda and mirbase.The targeting function analysis and signaling pathway analysis for the predicted target genes were completed with the help of GO-analysis.RESULTS:Compared with the normal group,there were 41 miRNAs genes up-regulated and 124 miRNAs genes down-regulated in the deep second-degree group,and greater differences were shown in some genes,including miR-210-3P,miR-99a-5P,miR-99b-3P,miR-99b-5P,miR-143-5P,miR-29c-5P,miR-15b-5P,miR-323a-3P,miR-424-5P,miR-125b-1-3P,miR-125a-3P,miRNA-184,which were involved in the inflammatory response,immune response and the healing process.CONCLUSION:The regulatory mechanism of miRNA with differential expression and its corresponding target genes in the healing of burn wounds was confirmed at the gene level,which provided targets for the precise treatment for burn wounds.