杉木涩籽形成过程的花青素还原酶基因表达

ANR gene expression during the formation of sterile seeds of Cunninghamia lanceolata

为探究杉木涩籽形成过程中,花青素还原酶(ANR)基因在类黄酮生物合成途径中的分子调控机制,以种子为试验材料,通过RT-PCR结合RACE的方法对杉木ANR基因进行全长克隆,运用q PCR技术分析不同涩籽率的杉木家系中ANR基因在种子不同发育时期的表达模式。结果显示,克隆到的ANR基因全长c DNA序列为1 357 bp,具有一个1 056 bp的开放阅读框,编码351个氨基酸。保守域预测显示ANR蛋白属于SDR超家族,具有一个FR_SDR_e特异性结构域。q PCR结果发现,在高涩籽率杉木中,90、100 d的表达量一直显著高于中、低涩籽率杉木;而在中、低涩籽率杉木中,ANR基因的表达模式仅仅是种子发育80 d时较高,之后明显下调。表明杉木涩籽率可能与其ANR基因表达相关。

To explore the role of anthocyanin reductase(ANR)gene in flavonoid biosynthesis pathway during the formation of sterile seeds of Chinese fir,the full-length ANR gene was cloned by RT-PCR combined with RACE,and the ANR gene expression in different development stages of seeds was analyzed by qPCR.The results showed that the full-length cDNA sequence of the cloned ANR gene was1357bp,it contains a1056bp open reading frame and encodes351amino acids.Conservative domain prediction indicated that ANR protein belongs to SDR superfamily and has a FR_SDR_e specific domain.qPCR results showed that ANR gene expression in family with high sterile seed rate was significantly higher at90and100d of seed development than the families with middle and low sterile seed rate.However,the ANR gene expression in families with middle and low sterile seed rate was only higher at80d of seed development,and then decreased.The results showed that the sterile seed rate of Chinese fir might be related to ANR gene expression.