黑木相思组培苗瓶外生根技术研究

Test on ex vitro rooting technology of tissue culture seedlings of Acacia melanoxylon

以黑木相思优良无性系组培苗为材料,开展了移栽基质与基质含水率,生根促进剂种类、浓度和处理时间,光照强度和光周期对组培苗瓶外扦插生根影响的研究,探讨组培苗瓶外生根的最佳条件,以期为黑木相思快速繁殖技术提供一定理论支撑和技术支持。结果表明:移栽基质以泥炭土+蛭石+珍珠岩(5∶4∶1)效果最好,其平均生根率达94.45%;含水率以50%效果最佳;生根促进剂以ABT(50 mg·L^(-1)处理25 min)表现最好,其平均生根数、平均根长、平均生根率分别为11.05条、1.67 cm、98.66%;光照强度对黑木相思组培苗瓶外生根影响显著,在光照强度为45～135μmol·m^(-2)·s^(-1)内,随着光照强度的增加,平均生根数与平均生根率随之增加,而平均根长呈下降趋势;光周期对黑木相思组培苗瓶外生根的影响不显著。

Tissue culture seedlings of Acacia melanoxylon were used as materials,the following factors on the ex vitro rooting influence of tissue culture seedings,including transplant substrate and substrate water content,type of rooting promoter,concentration and processing time,the light intensity and photoperiod were studied to explore the best conditions for ex vitro rooting of A.melanoxylon and provide some theoretical and technical support for the rapid breeding technology of A.melanoxylon.The results showed that:the graft substrate of peat+vermiculite+perlite(5∶4∶1)was the best,with an average rooting rate of94.45%;the effect of water content of50%was the best;the effect of rooting promoter of ABT(50mg·L^-1,25min)was the best with the average root number,the average number of rooting,the average root length and the average rooting rate were11.05,1.67cm and98.66%,respectively.Different illumination intensity had a great influence on ex vitro rooting of A.melanoxylon seedling.When the intensity of illumination was45^-135μmol·m^-2·s^-1,and the intensity of light increased,the average number of rooting and the average rooting rate increased with the increase of light intensity,but the average root length was decreasing;the effect of photoperiod on the ex vitro rooting of A.melanoxylon was not significant.