混合培养对益生菌和小球藻生长的影响

Effects of Mixed Cultivation on the Growth of Probiotics and Chlorella pyrenoidosa

将海水小球藻分别与光合细菌、枯草芽孢杆菌、乳酸菌混合进行培养,测量混合培养条件下小球藻和菌的生长情况,分析藻和菌之间的相互影响。结果表明:小球藻与光合细菌混合培养,小球藻持续增长,增殖速度显著快于其他各组,培养到第7天时,密度达到4. 134×10~7cells/mL,平均日增长率为30. 2%;小球藻分别与枯草芽孢杆菌和乳酸菌混合培养,前5天对小球藻的增长没有显著影响,第6天后小球藻的增长均受到抑制;小球藻与菌混合培养和菌单独培养这两种条件下菌的增殖速率没有显著差异。结论:菌藻混合培养中,光合细菌对小球藻的生长具有显著的促进作用,而枯草芽孢杆菌和乳酸菌在前期不影响小球藻生长,但是在后期培养中对小球藻的生长具有显著的抑制作用;混合培养对益生菌的生长影响不显著。

The growth of Chlorella pyrenoidosa and probiotics were investigated when C.pyrenoidosa was cultured with Photosynthetic bacteria(PSB),Bacillus subtilis and Lactobacillus sp..The results were as follows:The growth of C.pyrenoidosa was sustained when it cultivated with PSB,and the proliferation rate of C.pyrenoidosa was significantly faster than other groups.The concentration of C.pyrenoidosa was the highest(4.134×10^7cells/mL)at the7th day,and the average daily proliferation rate was30.2%;Mixed cultivation of C.pyrenoidosa with B.subtilis or Lactobacillus sp.had no significant effect on the growth of C.pyrenoidosa at the previous5d,but the growth of C.pyrenoidosa was inhibited after the6th day.The proliferation rate of probiotics was not significantly different between experimental group and control group.Conclusion:The mixed cultivation of C.pyrenoidosa and PSB could promote the growth of C.pyrenoidosa;B.subtilis and Lactobacillus sp.did not affect the growth of C.pyrenoidosa in the early stage,but they had a significant inhibitory effect on the growth of C.pyrenoidosa in the later;while the mixed cultivation of C.pyrenoidosa and probiotics had no significant effect on the growth of probiotics.