人卵巢组织冷冻及异种移植对始基卵泡凋亡的影响

Effects of freezing and xenotransplantation of human ovarian tissue on the apoptosis of primordial follicle

目的:通过检测始基卵泡凋亡率的改变来探讨2种冷冻方法对人卵巢组织的冻融及异种移植后的效果。方法:共收集16例人卵巢组织标本,每例分成2份,其中1份应用程序化冷冻方法进行冷冻,另一份应用玻璃化冷冻方法进行冷冻。解冻后分别移植到去卵巢的雌性SCID裸鼠颈部皮下。32只裸鼠随机分为2组,程序化冷冻组移植以程序化方法冷冻复苏的人卵巢组织;玻璃化冷冻组移植以玻璃化方法冷冻复苏的人卵巢组织。解冻后及移植6周后观察2组卵巢组织中始基卵泡的形态学改变及凋亡情况。结果:解冻后,程序化冷冻组始基卵泡凋亡率明显低于玻璃化冷冻组,始基卵泡正常率2组相比无明显差别。移植后,始基卵泡正常率及凋亡率2组相比均无明显差别。无论是程序化冷冻组还是玻璃化冷冻组,解冻后和移植后相比,始基卵泡正常率及凋亡率均无明显差别。结论:与玻璃化冷冻相比,程序化冷冻更有利于人卵巢组织始基卵泡的存活。将解冻后的人卵巢组织进行异种移植不影响始基卵泡的凋亡。

To investigate the effect of thawing and xenotransplantation of two kinds of cryopreservation methods on human ovarian tissue by detecting the apoptosis of primordial follicles.Methods:16 cases of human ovarian tissue specimens were collected,and each case was divided into two parts.One part was cryopreserved by programmed freezing and the other one was cryopreserved by vitrification.32 SCID nude mice were divided into two groups.Programmed freezing group transplanted the thawed human ovarian tissue frozen by programmed freezing,and vitrification group,by vitrification.After thawing and 6 weeks after transplantation,the survival rate and the apoptosis rate of the primordial follicles were observed in the two groups.Results:After thawing,the apoptosis rate of primordial follicles in programmed freezing group was significantly lower than that in vitrification group,and there was no significant difference in the normal follicular rate between the two groups.After transplantation,there were no significant differences in the survival rate and apoptosis rate of primordial follicles between the two groups.In programmed freezing group and vitrification group,there were no significant differences in the survival rate and the apoptosis rate of primordial follicles after thawing and after the transplantation.Conclusion:Programmed freezing is more beneficial to the survival of primordial follicles in the human ovarian tissue than vitrification.The heterograft of thawed human ovarian tissue does not affect the apoptosis of primordial follicles.