FGF3和FGF10对人卵巢癌SKOV3细胞迁移和侵袭的影响及其与Wnt/β-catenin信号通路的关系

Influences of FGF3 and FGF10 on Migration and Invasion of Human Ovarian Cancer SKOV3 Cells and Relation Between These Influences and Wnt/β-catenin Signaling Pathway

目的探讨FGF3和FGF10对人卵巢癌SKOV3细胞株迁移和侵袭的影响及其与Wnt/β-catenin信号通路的关系。方法体外培养人卵巢癌SKOV3细胞,采用人工合成siRNA干扰细胞的FGF3和FGF10基因,运用Real-time PCR和Western blot分别从mRNA水平和蛋白水平检测干扰效果。Transwell检测FGF3和FGF10蛋白表达下调后细胞迁移和侵袭能力的变化。采用LiCl激活Wnt/β-catenin信号通路,运用Real-time PCR检测FGF3和FGF10基因的变化。结果 FGF3和FGF10基因沉默组细胞迁移和侵袭能力与阴性对照(NC)组细胞、未处理组SKOV3细胞相比明显减弱(P<0.05),NC组与未处理组SKOV3细胞迁移和侵袭能力差异无统计学意义(P>0.05)。激活Wnt/β-catenin信号通路后,FGF3和FGF10基因水平增高。结论 FGF3和FGF10基因受Wnt/β-catenin信号通路的调控,促进人卵巢癌SKOV3细胞的迁移和侵袭。

Objective To explore the influences of FGF3 and FGF10 on migration and invasion of human ovarian cancer SKOV3 cells and relation between these influences and Wnt/β-catenin signaling pathway.Methods Human ovarian cancer SKOV3 cells were cultured in vitro and treated by FGF3 and FGF10 synthetic small interfering RNA.Then we used real-time PCR and Western blot method to detect the interference effect.And then we utilized Transwell assay to detect the changes of migration and invasion of human ovarian cancer SKOV3 cells after down-regulating FGF3 and FGF10 gene expression.Moreover,we exploited real-time PCR method to detect changes of FGF3 and FGF10 gene after activating Wnt signaling pathway by LiCl.Results Knockdown of FGF3和FGF10 could reduce the migration and invasion of SKOV3 cells,compared with NC group and untreated SKOV3 group(P<0.05).The migration and invasion abilities of NC group did not change significantly,compared with untreated SKOV3 group(P>0.05).FGF3 and FGF10 expression levels were increased significantly after activating the Wnt/β-catenin signaling pathway.Conclusion FGF3 and FGF10 modulated by Wnt/β-catenin signaling pathway promote the migration and invasion abilities of human ovarian cancer SKOV3 cells.