血清半乳凝集素3介导心房颤动机制的相关研究

Role of Galectin-3 in atrial fibrillation

目的研究心房颤动(AF)患者血清半乳凝集素3(Gal-3)水平与心肌纤维化指标的关系,探讨Gal-3介导心房颤动的机制。方法选取盛京医院心血管内科住院心功能正常的40例房颤患者作为房颤组,同期在选取本院20例健康体检者作为对照组。采用酶联免疫吸附法(ELISA)检测患者血清中Gal-3、转化生长因子β_1(TGF-β_1)、Smad3蛋白及I型前胶原羧基端肽(PICP)浓度;比较两组各指标差异,并分别对Gal-3与其他指标进行相关性分析。结果与对照组比较,房颤组Gal-3、TGF-β_1、Smad3、PICP增高(P <0.05);房颤组Gal-3与PICP、Gal-3与TGF-β_1、TGF-β_1与Smad3及TGF-β_1与PICP均呈正相关(r=0.695、0.760、0.890及0.829,均P=0.001)。结论房颤患者血清Gal-3及心肌纤维化指标水平增高,Gal-3可通过加速TGF-β_1/Smad3信号通路的传导促进心肌纤维化,最终导致心房颤动的发生。

Objective To study the relationship between Galectin-3(Gal-3)and atrial fibrillation(AF)in patients with atrial fibrillation and potential mechanisms.Methods A total of 40 AF patients who had normal cardiac function were selected as AF group,and 20 healthy subjects were enrolled as control group.Levels of Gal-3,transforming growth factor-beta 1,Smads protein and precollagen type I carboxy terminal peptide were measured by enzyme-linked immunosorbent assay.Correlation between Gal-3 and other parameters was analyzed.Results Levels of Gal-3,TGF-β1,Smad3 and PICP in AF group were increased significantly compared with those in control group(P<0.05).Statistical analysis showed that there was a significant positive correlation between Gal-3 and PICP,Gal-3 and TGF-beta 1,TGF-beta 1 and smad3,and TGF-beta 1 and PICP in AF group(r=0.695,0.760,0.890 and 0.829;all P=0.001).Conclusions Gal-3 and myocardial fibrosis indices in AF patients increase and Gal-3 may promote myocardial fibrosis by activating TGF-beta 1/Smad3 signal pathway.