免疫因子FoxP3、CCL22和CCR4在宫颈癌微环境中的调节作用

The investigation of regulatory role of immune factors FoxP3,CCL22 and CCR4 in the microenvironment of cervical cancer

目的:了解FoxP3、CCL22和CCR4 mRNA在宫颈癌组织中的表达情况,探究各因子的免疫调节作用及对肿瘤的影响。方法:采用实时定量聚合酶链反应检测30例宫颈癌癌灶、癌旁和20例正常宫颈组织中,FoxP3、CCL22和CCR4 mRNA表达水平。结果:与正常宫颈组织相比,FoxP3和CCL22 mRNA在癌灶和癌旁组织中的表达水平增高,CCR4 mRNA在癌灶中表达水平较高,FoxP3和CCL22在HPV阳性的宫颈癌灶和癌旁组织中的表达高于HPV阴性组,CCR4在HPV感染阳性和阴性组中的表达未见统计学差异。三者mRNA在高级别鳞癌的癌灶及癌旁组织中的表达水平均高于低级别鳞癌,且三者的mRNA表达水平在宫颈癌灶和癌旁组织中均两两正相关。结论:在宫颈癌微环境中,免疫因子FoxP3、CCL22和CCR4可能下调宫颈局部的免疫效应,同时三者互相协同加剧免疫失衡,促进宫颈癌的发生。

Objective: To explore the expression and correlation of FoxP3,CCL22 and CCR4 in cervical carcinoma,therefore to dig their immunomodulatory role in local site of cervical cancer. Methods: Totally 30 cases of cervical carcinoma with adjacent tissues and 20 cases of normal cervix tissues were enrolled in this study. Real-time Polymerase Chain Reaction was performed to profile the mRNA expression pattern of FoxP3,CCL22 and CCR4 in these samples. Results: FoxP3 and CCL22 mRNAs expression in normal cervix was lower than that in neoplastic foci and pericarcinous tissues. The expression level of CCR4 mRNA in neoplastic foci was higher than that in pericarcinous tissues and normal cervix. The mRNA expression of FoxP3 and CCL22 was increased in HPV-positive neoplastic foci and pericarcinous tissues while CCR4 seemed invariable regardless of different HPV infection status. Compared to low grade squamous cell carcinoma,the mRNA of FoxP3,CCL22 and CCR4 increased moderately in high grade leisions.The pair wise positive correlations were exhibited in both neoplastic foci and pericarcinous tissues among the mRNA expression level of these three factors. Conclusion: In microenvironment of cervical cancer,FoxP3,CCL22 and CCR4 may exert an influence on down-regulating immune response and the positive relevance among these three factors implies that they may synergistically promote immunosuppression,thus deteriorating the disequilibrium of local immune status.