不同采摘时间干、鲜道真洛龙党参挥发油的GC-MS分析

Analysis of Volatile Oil in Different Picking Time of Dried/Fresh Daozhen Luolong Codonopsis by GC-MS

研究不同采摘时间干、鲜道真洛龙党参挥发油成分的变化。采用水蒸气蒸馏的方法分别对干、鲜道真洛龙党参中的挥发油成分进行提取,提取物采用气相色谱-质谱联用技术(GC-MS)进行分析鉴定,以面积归一法计算各组分的相对百分含量。干道真洛龙党参挥发油主要成分为棕榈酸、亚油酸、肉豆蔻酸、棕榈酸甲酯、亚油酸甲酯和角鲨烯等。鲜道真洛龙党参挥发油主要成分为角鲨烯、亚油酸甲酯、棕榈酸、14-甲基十五烷酸甲酯和1-柠檬烯等。11月中旬采摘的干、鲜道真洛龙党参共有挥发油成分含量最高。干和鲜道真洛龙党参挥发油成分的种类差异不大,但含量差异较为明显。不同采摘时间干、鲜道真洛龙党参共有挥发油成分差异均不大,但其余挥发油成分差异明显。本研究首次对道真洛龙党参挥发油成分进行分析报道,以期为道真洛龙党参进一步开发研究提供参考。

To investigate the changes in volatile oil components of dried and fresh Daozhen Luolong Codonopsis in different picking time.The volatile oil components of dried and fresh Daozhen Luolong Codonopsis were extracted via the steam distillation method and identified by gas chromatography-mass spectrometry(GC-MS)technology.The relative percentage of each component was calculated by peak area normalization method.The main volatile oil components of dried Daozhen Luolong Codonopsis were palmitic acid,linoleic acid,myristic acid,methyl hexadecanoate,octadecadienoic acid methyl ester,and squalene.The main volatile oil components of fresh Daozhen Luolong Codonopsis were squalene,octadecadienoic acid methyl ester,palmitic acid,14-methyl-pentadecanoic acid-methyl ester,and l-limonene.The common volatile oil components of dried and fresh Daozhen Luolong Codonopsis collected in the middle of November were the highest.The types of volatile oil components in dried and fresh Daozhen Luolong Codonopsis were similar,but their contents were obviously different.The common volatile oil components of dried and fresh Daozhen Luolong Codonopsis in different picking times were similar,but there were significant differences in the other volatile oil components.In this study,the volatile oil components of Daozhen Luolong Codonopsis were analysised and reported for the first time,in order to provide reference for the further development and research of Daozhen Luolong Codonopsis.