两种柳珊瑚Anthogorgia caerulea和Menella kanisa的化学成分及其抗海洋污损生物附着活性研究

Chemical Constituents from Guangxi Beibu Gulf Gorgonian Menella kanisa and Anthogorgia caerulea and Their Antifouling Activity

采用多种色谱分离技术,从北部湾的两种柳珊瑚Anthogorgia caerulea和Menella kanisa分离出7种次级代谢产物,运用MS、1H NMR和13C NMR等波谱方法并结合文献对照分别鉴定为N-(1-羟甲基-2-羟基)3,6-二烯-十七烷基-十六脂肪酸酰胺(1),邻苯二甲酸二丙酯(2),3-(2-苯乙基)苯酚(3),对甲氧基苯甲酸甲酯(4),邻苯二甲酸二己酯(5),梾木甙(6),次黄嘌呤核苷(7),其中化合物1为新化合物,其他化合物均首次从小月柳珊瑚(Menella kanisa)和花柳珊瑚(Anthogorgia caerulea)中分别分离得到。除化合物3,7外均显示出抗海洋污损生物藤壶幼虫附着能力,其中以化合物6的活性最好,化合物1的活性次之,其抗藤壶幼虫附着EC50分别为6. 89,8. 72μg/m L。

To study the chemical constituents and their antifouling activity from two gorgonian Anthogorgia caerulea and Menella kanisa were isolated from Beibu gulf,Guangxi.Seven compounds were purified by means of chromatographic techniques and their structures were identified on the basis of spectral features,which were identified respectively as N-[(1-hydroxymethyl)-2-hydroxy-3,6-heptadecadienyl]-hexadecanamide(1),dipropyl phthalate(2),3-(2-phenylethyl)phenol(3),methyl ester of p-methoxybenzoicacid(4),dihexyl phthalate(5),cornoside(6),inosine(7).1was a new compound,and others were isolated from Menella kanisa and Anthogorgia caerulea for the first time,respectively.Antifouling activity was performed by the antilarval(cyprids of barnacle Balanus amphitrite)settlement bioassay.All compounds with exception of3and7showed significant antifouling activity against the barnacle larvae.Especially,N-[(1-hydroxymethyl)-2-hydroxy-3,6-heptadecadienyl]-hexadecanamide and cornoside were better potent antifouling metabolites with EC50values of8.72and6.89μg/mL,respectively.