益母草抗结核分枝杆菌成分的跟踪分离

Isolation and Identification of Anti-Mycobacterium tuberculosis Components from Leonurus artemisia(Laur.) S.Y.Hu F

结核病分为肺结核和肺外结核,其中子宫结核属于肺外结核,是造成不孕的主要原因。益母草是我国传统中药,常用于妇科疾病的治疗。在筛选中发现,益母草乙醇提取物具有抗结核分枝杆菌活性,经活性跟踪分离,从益母草乙酸乙酯萃取部位中分离得到具有抑制结核分枝杆菌的单体化合物1,鉴定为益母草碱,抗结核杆菌的MIC值为80μg/m L。这一发现为治疗子宫结核提供了新的线索。

Tuberculosis is classified as pulmonary tuberculosis and extrapulmonary tuberculosis.The endometrial tuberculosis belongs to extrapulmonary tuberculosis,which is the main cause of infertility.Leonurus artemisia(Laur.)S.Y.Hu F is a traditional Chinese medicine,commonly used in the treatment of gynecologic diseases.In screening,it was found that the ethanol extract of Leonurus artemisia(Laur.)S.Y.Hu F had anti-Mycobacterium tuberculosis activity.Based on bioassay-guided isolation,compound1with the activity of inhibiting Mycobacterium tuberculosis was separated from the ethyl acetate extracts of Leonurus artemisia(Laur.)S.Y.Hu F.It was identified as leonurine,and with MIC value of80μg/mL for against tubercle bacillus.This discovery might provide a new clue for the treatment of uterine tuberculosis.