摘要
探究不同浓度木糖及补料对树干毕赤酵母(Pichia stipitis)菌株1K-9发酵木糖产乙醇的影响,提高木糖产乙醇的发酵水平,为扩大规模发酵木糖产乙醇打下基础。结果表明,菌株1K-9先采用10%木糖进行乙醇发酵,36 h补加与10%木糖培养基等体积的20%木糖培养基继续发酵,发酵至108 h时菌数也达到了(12.16±0.07)×10~8个/m L,较未补料发酵时有所提高;发酵108 h时醪液中残留的木糖含量为(1.03±0.02)g/L,较未补料发酵时有所降低;乙醇含量达到了6.56%vol,较未补料时提高了1.85%vol。因此补料发酵是有效的。
The effect of different concentration of xylose and fed-batch fermentation on the ethanol production of Pichia stipitis1K-9from xylose was investigated,to improve the level of ethanol production and to lay a foundation for increasing the ethanol production from xylose fermentation.The results indicated that strain1K-9was supplemented with10%xylose for ethanol fermentation,and was fed-batch with20%xylose medium(the same volume of10%xylose medium)after36h fermentation.The microbial count was(12.16±0.07)×10^8CFU/ml at108h,which was increased than that before fed-batch fermentation.The residual xylose content in the mash was(1.03±0.02)g/L,which was decreased than that before fed-batch fermentation.The ethanol content reached6.56%vol,which was1.85%vol higher than that before fed-batch fermentation.Therefore,supplementary fermentation was effective.
作者
吴仁智
陈东
黄俊
陆琦
芦志龙
陈英
陈小玲
黄日波
WU Renzhi;CHEN Dong;HUANG Jun;LU Qi;LU Zhilong;CHEN Ying;CHEN Xiaoling;HUANG Ribo(National Engineering Research Center for Non-food Biorefinery, State Key Laboratory of Non-food Biomass Enzyme Technology, Guangxi Biomass Industrialization Engineering Institute, Guangxi Key Laboratory of Biorefinery, Guangxi Academy of Sciences, Nanning 530007, China;College of Life Science and Technology, State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning 530004, China)
出处
《中国酿造》
CAS
北大核心
2018年第12期112-115,共4页
China Brewing
基金
国家863项目(2013AA050701)
广西自然科学基金联合资助培育项目-面上项目(2018GXNSFAA138111)
广西重点研发计划(桂科AB16380024)
广西科技开发项目(桂科合14123001-6)
广西科学院基本科研业务费(12YJ25SW04)
广西科学院科研发展基金项目(2018YFJ402)
关键词
树干毕赤酵母
乙醇
木糖
补料发酵
Pichia stipitis
ethanol
xylose
fed-batch fermentation