“三法三穴”推拿手法对坐骨神经损伤大鼠运动功能和脊髓NogoA及其受体表达的影响

Effects of Tuina of Three Handing-Three Points on Motor Function and Expression of NogoA and Its Receptor in Spinal Cord of Rats with Sciatic Nerve Injury

目的探讨"三法三穴"推拿手法对坐骨神经损伤大鼠后肢运动功能和脊髓中NogoA及其受体NgR表达的影响。方法 64只雄性Sprague-Dawley大鼠随机分为正常组(n=16)、假手术组(n=16)、模型组(n=16)和推拿组(n=16)。模型组和推拿组采用夹持法制备坐骨神经损伤模型,推拿组以按摩推拿手法模拟仪模拟点、拨、揉三种手法按摩殷门、承山、阳陵泉三穴。分别于术后7 d和干预20 d行斜板测试;取大鼠脊髓组织,Westen blotting检测NogoA和NgR蛋白表达。结果术后7 d,与正常组比较,模型组和推拿组斜板测试评分降低(P <0.05),NgR表达升高(P <0.05)。干预20 d,与模型组比较,推拿组斜板测试评分升高(P <0.05),NgR表达降低(P <0.05),接近正常组水平(P> 0.05)。结论 "三法三穴"推拿手法能改善坐骨神经损伤大鼠后肢运动功能,可能与抑制脊髓中NgR蛋白表达有关。

Objective To investigate the effects of Tuina of Three Handing-Three Points on hindlimb motor function and expression of protein NogoA and its receptor NgR in the spinal cord of rats with sciatic nerve injury.Methods A total of 64 male Sprague-Dawley rats were randomly assigned to normal group(n=16),sham group(n=16),model group(n=16)and Tuina group(n=16).The model group and Tuina group prepared the sciatic nerve injury model with brace method.Tuina group received Tuina in methods of pressing,strumming and circular rubbing on Yinmen(BL37),Chengshan(BL57)and Yanglingquan(GB34)with Tuina manipulation emulator.The rats were assessed with oblique plate test seven days after operation and 20 days after Tuina.Then,the spinal cord tissues were extracted to detect the expression of protein of NogoA and NgR with Western blotting.Results The scores of the oblique plate test decreased in the model group and Tuina group seven days after operation compared with those in the normal group(P﹤0.05),while the expression NgR increased(P﹤0.05).The scores of the oblique plate test increased in Tuina group compared with those in the model group 20 days after Tuina(P<0.05),while the expression of NgR decreased(P﹤0.05),similar to the normal group(P>0.05).Conclusion Tuina of Three Handing-Three Points can improve the hindlimb motor function in rats with sciatic nerve injury,which may be related to inhibition of the expression of NgR in the spinal cord after sciatic nerve injury.