棕鞭藻及其培养滤液对铜绿微囊藻生长及生理特性的影响

EFFECTS OF OCHROMONAS SP. CULTURE MEDIA FILTRATE ON THE GROWTH AND PHYSIOLOGICAL TRAITS OF MICROCYSTIS AERUGINOSA

为探究藻类之间的可能存在的信息传递,研究了棕鞭藻(Ochromonas sp.)及其培养滤液对铜绿微囊藻的生长及生理特性的影响。结果发现, 3种不同接种比例(1﹕4、1﹕1和4﹕1)的棕鞭藻与微囊藻共培养下,微囊藻细胞密度到第4天均下降到最低值,而棕囊藻细胞密度则显著增加。同时,棕鞭藻培养滤液能够抑制微囊藻的生长、导致丙二醛(MDA)含量和过氧化氢酶(CAT)活性。此外,棕鞭藻培养滤液也能促进微囊藻胞外多糖(EPS)含量显著增加。这表明棕鞭藻不仅能吞噬微囊藻,而且可能释放某些化感物质抑制微囊藻生长及生理参数。这暗示了棕鞭藻可作为潜在的藻类水华控制生物,抑制早期藻类大量增殖。

Microcystis bloom has caused many environmental problems,and its mechanism of bloom-formation and adaptation has been noticed wildly.To examine the possible information transfer by chemicals between both algae,the effect of Ochromonas and its culture media filtrate on the growth and physiological traits of Microcystis aeruginosa was investigated in this study.The results indicated that the cell density in Microcystis decreased significantly,while the cell density in Ochromonas increased markedly when Microcystis and Ochromonas were co-cultured at three initial cell density(1﹕4,1﹕1 and 4﹕1).Moreover,the culture media filtrate of Ochromonas sp.could inhibit the growth,and resulted in an increase of malondialdehyde(MDA)content and catalase(CAT)activity.Additionally,the content of extracellular polysaccharides(EPS)increased markedly in Microcystis when it was inoculated into the culture media filtrate of Ochromonas sp.These results indicated that Ochromonas not only could prey on Microcystis,but also might release some of the chemicals to inhibit the growth and physiological parameters of Microcystis.This suggested that Ochromonas might be used to inhibit the early proliferation of algae as a potential control of algae blooms.