烟草Nt CIPK家族基因表达与其含钾量之间的关系分析

Analysis of the Relationship Between NtCIPK Family Gene Expression and Potassium Content in Tobacco

为探讨烟草NtCIPK家族基因的表达与烟草含钾量之间的关系,为烟草钾吸收的分子机制研究提供借鉴,对普通烟草品种K326、贵烟5号和云87进行3个不同钾浓度(6. 0,1. 0,0. 2 mmol/L)的溶液培养,并测定其干质量和钾含量;同时,运用qRT-PCR的方法,分析了NtCIPK家族11个基因对不同钾浓度处理的诱导表达模式。结果表明,随着培养液钾浓度的提高,各参试品种的单株干质量和钾含量都呈升高趋势,且处理间有显著差异,其中,云87在不同钾浓度(1. 0,0. 2 mmol/L)处理下,其单株干质量和钾含量均最高。NtCIPK家族11个基因的表达均受钾浓度变化的诱导,表现为随着K+浓度的下降而上调表达;只有K326的NtCIPK9和贵烟5号的NtCIPK24的表达呈现下调趋势。通径分析结果表明,在0. 2 mmol/L K+浓度处理下,NtCIPK3和NtCIPK5的表达分别与K326和贵烟5号的单株含钾量呈现显著正相关关系;而在6. 0 mmol/L K+浓度处理下,NtCIPK23的表达与云87的单株含钾量呈现显著负相关关系。烟草钾含量与其NtCIPK基因的表达密切相关,而NtCIPK基因的增强表达有助于烟草对钾的吸收和积累。

To explore the relationship between tobacco NtCIPK family gene expression and the potassium content of tobacco,and to provide reference for the molecular mechanism of potassium absorption in tobacco.For common tobacco varieties K326,Guiyan5and Yun87three different potassium concentrations(6.0,1.0,0.2mmol/L)were cultured in solution and their dry weight and potassium content were measured.At the same time,11genes of the NtCIPK family were analyzed for different potassium concentrations using qRT-PCR.Induced expression pattern.With the increased of potassium concentration in the culture medium,the dry weight and potassium content of the tested plants showed an increasing trend,and there were significant differences between treatments.Among them,Yun87is treated with different potassium concentrations(1.0,0.2mmol/L).Dry weight and potassium content were the highest.The expression of11genes in the NtCIPK family was induced by the change of potassium concentration,and showed up-regulated expression with the decreased of K^+concentration;only the expression of NtCIPK9in K326and NtCIPK24in Guiyan5showed a downward trend.The results of path analysis showed that the expression of NtCIPK3and NtCIPK5was significantly positively correlated with the potassium content of K326and Guiyan5,respectively,at the concentration of0.2mmol/L K^+,while NtCIPK23was treated at the concentration of6.0mmol/L K^+.The expression had a significant negative correlation with the potassium content per plant of Yun87.The potassium content of tobacco was closely related to the expression of NtCIPK gene,and the enhanced expression of NtCIPK gene contributes to the absorption and accumulation of potassium by tobacco.