弱光胁迫下鄞红葡萄生理生化特性及相关基因的研究

Effects of Weak Light Stress on Physiological and Biochemical Characteristics and Relative Gene Expression of Yinhong Grape

弱光胁迫下葡萄的生理特性及该环境下相关基因的表达一直缺乏研究,植物细胞会因弱光胁迫造成细胞膜系统和代谢过程的损伤,选择不同遮阴处理对葡萄进行弱光响应的相关研究,旨在为生产上该品种的抗弱光性能提供理论依据。以1年生鄞红葡萄进行盆栽试验,采用不同光照强度(0,25%,40%,70%,85%)遮阴处理,验证不同胁迫环境下该葡萄品种的生长特性、细胞保护酶活性、渗透调节物质含量变化,并且对抗逆基因MYC2、TRX进行Q-PCR表达量分析。遮阴结束后,T1处理下各项生长及酶活指标良好,但随着弱光胁迫程度的增强植株生长受阻,SOD、POD、CAT、可溶性蛋白活性先升高后逐渐降低;而MDA和PRO活性与遮阴程度呈正相关; MYC2与TRX基因表达量在胁迫环境下先上调后下降。重度胁迫下(85%)葡萄的各项生理指标均下降显著,证明适度遮阴较利于鄞红生长,但重度胁迫会对植株造成不可逆损害,自身已无法通过调节细胞保护酶及合成相关基因以适应弱光胁迫。根据以上试验结论对葡萄耐弱光品种的选育进行了讨论,并认为今后需验证更多与弱光应答相关的基因,确认其是否受到弱光诱导。

The stress response and its physiological and relative gene characteristics of grape under the weak light conditions remains unclearly due to the lacking of research.The plant cell membrane system and metabolic process is also affected by low-light environment.As studies on response to weak light stress could provide for prevention of weak light adversity.Low light stress on one year Yinhong grape as the tested material with pot experiment,which was used to examine their growth changes,protective enzymes,osmotic adjusting materials,gene expression of MYC2and TRX used Q-PCR under the different levels.Ranging from shading intensity of0to85%(0,25%,40%,70%,85%).The results showed as follows:the growth and enzyme activities indicated that they all good at shading of T1,while the plant growth was gradually constrained with the enhanced weak light stress,SOD,POD,CAT,soluble protein activity,MYC2and TRX gene expression increased firstly and then decreased gradually.At the same time,the activity of PRO and MDA was positively related with the degree of shade.The physiological indexes under higher intensity of shading(85%)were decreased significantly,which indicating that severe stress could cause irreversible damage by mediating itself and not adapt the weak light stress by regulating the cell protective enzymes and synthesizing the related genes.The conservation,the breeding of weak light varieties of grape was also discussed on the basis of the research results,it was also believed that more genes related to the weak light response need to be verified in the future to confirm whether they were induced by weak light.