复方人参皂苷纳米乳对OVA接种小鼠免疫功能的影响

Effect of Compound Ginsenoside and Levamisole Hydrochloride Nanoemulsion on Immune Function of Mice Inoculated with OVA

旨在研究口服复方人参皂苷纳米乳对抗原免疫效果的影响。将小鼠分为正常对照组、卵清白蛋白(OVA)对照组、空白纳米乳组、盐酸左旋咪唑纳米乳组、人参皂苷纳米乳组、人参皂苷-盐酸左旋咪唑水溶液组和复方人参皂苷纳米乳组(高、中、低剂量),皮下注射OVA抗原2次,在首次和第2次注射前3d连续灌胃给药,第2次注射后14d,测定血清(IgG、IgG1和IgG2a)抗体水平、脾细胞因子(IFN-γ和IL-4)水平以及脾淋巴细胞增殖刺激指数。结果表明,复方人参皂苷纳米乳中剂量(50mg·kg-1)组IL-4、IgG和IgG1水平显著或极显著高于复方人参皂苷纳米乳低剂量组和盐酸左旋咪唑纳米乳组,IFN-γ、IgG2a及OVA诱导脾淋巴细胞增殖刺激指数显著或极显著高于人参皂苷纳米乳组、人参皂苷-盐酸左旋咪唑水溶液组、盐酸左旋咪唑纳米乳组、复方人参皂苷纳米乳高剂量组和低剂量组。口服复方人参皂苷纳米乳能诱导OVA免疫小鼠产生Th1/Th2混合型免疫反应,表明人参皂苷和盐酸左旋咪唑能协同增强小鼠的细胞免疫和体液免疫功能。

To investigate the effect of compound ginsenoside and levamisole hydrochloride nanoemulsion(GS-LH-NE)on the immune effect of antigen,mice were divided into normal control group,OVA control group,blank nanoemulsion group,levamisole hydrochloride nanoemulsion(LH-NE)group,ginsenoside nanoemulsion(GS-NE)group,ginsenoside-levamisole hydrochloride(GS-LH)aqueous solution group,GS-LH-NE group(high dosage,medium dosage and low dosage).Mice were subcutaneously injected with antigen OVA twice and intragastrically administrated with drug for three days before the first and second injection.The levels of serum antibodies(IgG,IgG1,IgG2a),the levels of splenocyte cytokines(IFN-γ,IL-4)and proliferation stimulating index of splenic lymphocytes were measured at the14th day after the second injection.The results showed that the levels of IgG,IgG1and IL-4in GS-LH-NE medium dosage(50mg·kg^-1)group were significantly and extremely significantly higher than those in GS-LH-NE low dosage group and LH-NE group.The levels of IgG2a,IFN-γand proliferation stimulating index of splenic lymphocytes induced by OVA in GS-LH-NE medium dosage(50mg·kg^-1)group were significantly and extremely significantly higher than those in GS-NE group,GS-LH aqueous solution group,LH-NE group,GS-LH-NE high dosage group and low dosage group.Oral administration of GS-LH-NE could induce mixed Th1/Th2immune response in OVA-inoculated mice,indicating that ginsenoside and levamisole hydrochloride could synergistically enhance the cellular and humoral immune functions in mice.