Optimization of Ultrasound-assisted Enzymatic Polysaccharide Extraction from Turpiniae Folium Based on Response Surface Methodology

基于响应面法优化超声辅助提取山香圆叶多糖工艺（英文）

Objective To optimize the extraction procedure and obtain the maximum total polysaccharide yield from Turpiniae Folium.Methods Response surface methodology(RSM),combining Plackett–Burman design(PBD),steepest ascent method,and Box–Behnken design(BBD),was employed.The significant factors contributing to polysaccharide production were determined by PBD.The path of steepest ascent method was performed to rapidly reach the neighborhood of the optimum region.BBD and RSM were applied to further investigate the mutual interaction between the variables and to define the optimal values.Results The significant factors contributing to polysaccharide production were enzyme concentration,extraction time,and liquid-to-solid ratio.The optimal conditions for maximal TFP were:enzyme concentration of2.8%,extraction time of41min,and liquid-to-solid ratio of27mL/g.The experimental yield of3.08%was in good agreement with the model-predicted yield of3.1%under the optimized conditions.Conclusion This study was successfully applied to optimize the extraction conditions of Turpiniae Folium polysaccharide,which can contribute to its further production and application.

目的优化山香圆叶多糖提取工艺,获得最大多糖得率。方法应用响应面法联合Plackett-Burman设计、最陡爬坡法和Box-Behnken设计来获取山香圆叶多糖最佳提取工艺。利用Plackett-Burman设计筛选对多糖得率显著影响的因素,利用最陡爬坡法使各因素快速逼近最佳响应区域,采用Box-Behnken设计和响应面法来进一步研究各因素之间的相互作用,并得出最优值。结果 Plackett-Burman设计筛选出三个对多糖得率显著影响的因素:酶浓度、提取时间和液固比。在进一步研究中得到最大得率的最优条件为:酶浓度2.8%、提取时间为41 min和液固比为27 mL/g。在最优条件下山香圆叶多糖得率为3.08%,与预测值3.11%接近。结论本研究优化了山香圆叶多糖的提取工艺,有利于山香圆叶多糖后续的生产和利用。