摘要
利用PCR-SSCP技术对吉富罗非鱼HSP70基因多态性进行检测,并采用最小二乘法分析多态性与罗非鱼Oreochromis niloticus耐寒性状的相关性。结果发现,依据HSP70基因序列设计的引物HP70-1、HP70-4、HP70-6不同耐寒能力样本中的电泳图具有带型多态性,且该基因多态性与其耐寒性状显著相关。据此推断HSP70基因是潜在的罗非鱼耐寒相关基因。
The polymorphisms of HSP70in the GIFT tilapia samples were detected by using PCR-SSCP technique and the correlation with cold tolerance capability of GIFT tilapia was analyzed by least square method in this study.The results showed that the electrophoresis diagram of the primer HP70-1,HP70-4,HP70-6designed according to the HSP70has the polymorphism of the belt type in different cold tolerance samples.The polymorphism of the gene was significantly correlated with its cold tolerance.Therefore,it is inferred that HSP70genes are potential cold-resistant genes of tilapia.
作者
宾石玉
钟丹丹
杜雪松
张永德
林勇
黄姻
文露婷
BIN Shiyu;ZHONG Dandan;DU Xuesong;ZHANG Yongde;LIN Yong;HUANG Yin;WEN Luting(College of Life Science,Guangxi Normal University/Key Laboratory of Ecology of Rare and Endangered Speicies and Environmental Protection,Ministsry of Education,Guilin Guangxi 541006,China;Guangxi Academy of Fishery Sciences,Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture,Nanning Guangxi 530021,China)
出处
《广西师范大学学报（自然科学版）》
CAS
北大核心
2019年第1期211-217,共7页
Journal of Guangxi Normal University:Natural Science Edition
基金
广西科学研究与技术开发计划(桂科AB16380074)
国家现代农业产业技术体系广西罗非鱼创新团队建设项目(nycytxgxcxtd-08-01)
广西直属公益性科研院所基本科研业务费专项资金(GXIF-2014-016)
