老年乳腺癌患者外周血调节性T细胞的检测及意义

Detection of Regulatory T Cells in Peripheral Blood in the Elderly with Breast Cancer and the Significance

目的研究外周血调节性T细胞(Treg)在老年乳腺癌患者中的临床意义,探讨其与患者临床病理的相关性。方法选取2016年1月—2017年1月老年乳腺癌患者40例、老年乳腺良性病变者40例以及老年体检中心正常人群40例为研究对象,采用流式细胞术测定所选人群的Treg水平并比较;根据不同的临床病理将乳腺癌患者进行分组,比较Treg差异;对乳腺癌患者检测癌胚抗原(CEA)与糖类抗原(CA153),并比较Treg与二者之间的相关性。结果与乳腺良性病变组及正常人群组比较,乳腺癌组患者的Treg水平明显升高,差异显著有统计学意义(P<0.05);而乳腺良性病变组与健康对照组间Treg水平无显著差异(P>0.05)。Treg水平在肿瘤≥2 cm及淋巴结转移阳性组患者高于<2 cm患者及淋巴结转移阴性组,差异有统计学意义(P<0.05);在TNM分期上,Ⅲ、Ⅳ期组Treg水平高于Ⅰ、Ⅱ期组,差异有统计学意义。Treg阳性率43.33%,CA125阳率为21.67%;CA153阳性率为23.33%,Treg表达的阳性率明显高于CA125与CA153,差异有统计学意义(P<0.05)。结论 Treg在乳腺癌患者中高表达,抑制了抗肿瘤功能,与乳腺癌患者的分期进展存在着密切关系,提示其可以作为乳腺癌预测的一项指标。

Objective To explore the clinical significance of regulatory T cells(Treg)in peripheral blood in the elderly with breast cancer and to discuss its correlation with clinic pathology.Methods40cases of breast cancer(cancer group),40cases of benign breast lesions(benign group)and40normal cases of physical examination(normal group)during the period from Jan.,2016to Jan.,2017were selected;flow cytometry was applied in detecting the level of Treg in all the selected cases in the3groups;based on clinical pathological study,cases in cancer group were sub-divided and the differences in the level of Treg among the sub-groups were comparatively studied;routine examination of carcinoembryonic antigen(CEA)and carbohydrate antigen(CA153)were performed on cases in cancer group and the correlation of Treg with CEA and CA153was analyzed.Results The level of Treg in cancer group was much higher than that in benign group and in normal group(P<0.05)with statistical differences(P<0.05)while the level of Treg in benign group was of no difference from that in normal group(P>0.05);the level of Treg in cases with tumors>2cm and in cases with positive lymph node metastasis was significantly higher than that in cases with tumor<2cm and than that in cases with negative lymph node metastasis,the difference was of statistical significance(P<0.05);the level of Treg in cases of TNM stage III and IV was significantly higher than that in cases of TNM stageⅠandⅡ(P<0.05);the positive rate of Treg,CA125and CA153was43.33%,21.67%and23.33%,respectively;the positive rate of Treg expression was significantly higher than that of CA125and CA153(P<0.05).Conclusions The high expression of Treg in caseswith breast cancer inhabits the antitumor function and is of a close relationshipwith breast cancer staging,which suggests that Treg may be taken as an indicator for breast cancer prediction.