摘要
目的探讨类风湿关节炎(RA)患者外周血单个核细胞(PBMC)中凋亡相关蛋白Bcl-2与c-FLIP的表达水平及其临床意义。方法 60例类风湿关节炎患者外周血标本按RA疾病活动指数(DAS28)分为低度活动期(DAS28 <3. 2)、中度活动期(DAS28 3. 2~5. 1)与高度活动期(DAS28> 5. 1),以同期25例健康体检者外周血作为健康对照组(N组)。实时荧光定量PCR法检测RA患者PBMC中Bcl-2、c-FLIP、caspase-8凋亡相关蛋白的mRNA表达水平;蛋白免疫印迹法检测PBMC中cFLIP、caspase-8蛋白表达水平。结果 Bcl-2的低活动组mRNA相对表达量为1. 02±0. 35,高于健康对照组(0. 32±0. 18,P<0. 05),而中、高活动组分别为0. 08±0. 01,0. 05±0. 02,低于健康对照组(均P <0. 05)。c-FLIP的低、中、高三个活动组mRNA相对表达量分别为1. 27±0. 28,1. 32±0. 34,1. 93±0. 40,均高于健康对照组(1. 08±0. 12,均P <0. 05); caspase-8的低、中、高组mRNA相对表达量分别为2. 77±0. 97,4. 52±0. 85,2. 13±0. 44,也均高于健康对照组(均P <0. 05)。中活动组cFLIP蛋白表达明显高于健康对照组(P <0. 05),而低、高活动组与对照组差异无统计学意义(P> 0. 05);中、高活动组caspase-8蛋白表达明显高于健康对照组(P <0. 05),而低活动组表达差异无统计学意义(P> 0. 05)。结论 PBMC中凋亡相关蛋白c-FLIP mRNA表达水平随着RA疾病的进程增高,而Bcl-2只有低活动组有明显增高,此可为类风湿关节炎发病机制的后续研究提供参考。
Objective To investigate the expression and its significance of apoptosis-related proteins Bcl-2,c-FLIP and caspase-8in peripheral blood mononuclear cells of patients with rheumatoid arthritis(RA).Methods The blood samples of60patients with rheumatoid arthritis were divided into low activity(DAS28<3.2)group,moderate activity(3.2-5.1DAS28)group and high activity(DAS28>5.1)group according to the RA disease activity index(DAS28).The blood of25normal controls for health check-up were used as normal control group.The mRNA expression of apoptosis related proteins Bcl-2,c-FLIP and caspase-8was detected by realtime PCR,and the protein levels of c-FLIP and caspase-8were detected by Western blot.Results The mRNA expression of Bcl-2in low activity group was higher than in normal control group(P<0.05),but it was lower in moderate activity group and high activity group(P<0.05).The relative mRNA expression of c-FLIP in low activity group,moderate activity group and high activity group were higher than that in normal control group respectively(P<0.05).The expression level of Caspase-8mRNA was higher in low activity group,moderate activity group and high activity group than in normal control group(P<0.05).The protein level of c-FLIP in moderate activity group was significantly higher than that in control group(P<0.05),but there was no difference between low activity group and normal control group.The protein levels of Caspase-8in moderate activity group and high activity group were significantly higher than that in normal control group(P<0.05),but there was no difference between low activity group and normal control group.Conclusion In peripheral blood mononuclear cells of patients with RA,the relative mRNA expression level of c-FLIP is increased with the progress of the disease,while Bcl-2mRNA increases only in low activity patients compared with normal controls,but decreases in moderate and high activity patients.It provides reference for the further study of the pathogenesis of rheumatoid arthritis.
作者
林美娜
章涛
许瑞元
梅序桥
郭月丽
LIN Meina;ZHANG Tao;XU Ruiyuan;MEI Xuqiao;GUO Yueyli(Zhangzhou Health Vocational College,Zhangzhou 363000, China;Department of Immunology,Fujian Medical University;Department of Clinical Laboratory,Affiliated Zhangzhou Municipal Hospital, Fujian Medical University)
出处
《山西医科大学学报》
CAS
2018年第12期1488-1492,共5页
Journal of Shanxi Medical University
基金
福建省中青年教师教育科研项目(JAT160912)
漳州卫生职业学院院本课题(ZYZ201607)
漳州市科技专项基金资助项目(ZZ2018J41)