乙肝病毒标记物阳性表型血清标本HBV-DNA的检验分析

Analysis of HBV-DNA in serum samples of positive phenotype of hepatitis B virus marker

目的分析乙型病毒性肝炎中乙肝病毒标记物阳性表型血清标本HBV-DNA的临床诊断效果。方法选取2016～2017年本院900多例表面抗原阳性患者中的387例,全部患者均实施乙肝DNA检测,通过酶联免疫吸附试验(ELISA法)、FQ-PCR法定量分析乙肝病毒标记物阳性表型血清标本及其HBVDNA。结果 HBV-DNA阳性例数在疑似乙肝患者血清标本387例为187例,阴性200例,定量最高7.70E+009IU/mL、定量最低2.71E+003IU/mL。相比大三阳组,差异有统计学意义(P <0.05)。结论乙型病毒性肝炎HBVDNA含量经PCR技术检测后,诊断率较为可靠,为患者临床诊治提供了可靠有效途径。

Objective To analyze the clinical diagnostic effect of HBV-DNA in serum samples of positive phenotype of hepatitis B Virus marker in hepatitis B.Methods 387 of more than 900 patients with surface antigen positive in our hospital from 2016 to 2017 were selected.All patients were given DNA testing for hepatitis B.Serum samples of positive phenotype of hepatitis B Virus marker and its HBV-DNA were quantitatively analyzed by enzymelinked immunosorbent assay(ELISA)and FQ-PCR method.Results In 387 serum samples of suspected hepatitis B,the number of HBV-DNA positive cases was 187 and the number of negative cases was 200,with the highest quantitative value of 7.70E+009IU/mL and the lowest quantitative value of 2.71E+003IU/mL.Compared with the great three positive group,the difference was statistically significant(P<0.05).Conclusion The HBV-DNA content of hepatitis B virus is detected by PCR technology and the diagnostic rate is reliable,which provides a reliable and effective way for clinical diagnosis and treatment.