输注血小板患者血清皮质醇水平、血清肥大细胞脱颗粒与心理应激的相关性分析

An Analysis of the Correlation Between CS Levels,Mast Cell Degranulation and Psychological Stress in Patient with the Transfusion of Platelets

目的对输注血小板患者血清皮质醇(CS)水平、血清肥大细胞脱颗粒与心理应激的相关性做一系统分析。方法于2015年11月至2018年3月采用病例抽样的方法,抽取我院接受血小板输注治疗的患者,同时按照病例对照研究的原则选取不少于病例数量的对照(有效观察对象共100例,病例组49例,对照组51例);取患者空腹静脉血10mL,3000r/min离心取上清,采用ELISA法进行CS定量检测,采用血清标本涂片计数法计算血清肥大细胞脱颗粒率,采用大学生心理健康测评系统评估患者的心理应激状态。结果病例组的CS、血清肥大细胞脱颗粒率及心理应激水平均显著高于对照组(P<0.05);病例组男性的心理应激水平均显著高于女性(P <0.05);病例组随患者年龄的增大,其CS、血清肥大细胞脱颗粒率及心理应激水平均呈显著上升趋势(P<0.05);输注血小板患者的CS水平与血清肥大细胞脱颗粒率呈正相关(r=0.363,P <0.05),CS水平与心理应激水平呈正相关(r=0.490, P <0.001),血清肥大细胞脱颗粒率与心理应激水平呈正相关(r=0.472,P<0. 001)。结论输注血小板患者的CS水平、血清肥大细胞脱颗粒率与心理应激水平均呈正相关。

Objective To analyze the correlation between CS level,serum mast cell degranulation and psychological stress in patients with the transfusion platelet.Methods From November 2015 to March 2018,the method of case sampling was used to select patients who received the platelet transfusion in our hospital.At the same time,according to the principle of case-control study,about the same number of controls were selected.A total of 100 subjects were enrolled,49 cases in the case group,51 cases in the control group;We then took the patient′s fasting venous blood 10mL,centrifuged at 3000r/min to obtain the supernatant.ELISA method was used for CS quantitative detection,and serum sample smear counting method was used to calculate the serum hypertrophy.Using psychological health assessment system,we assessed the psychological stress state of patients.Results The CS and serum mast cell degranulation rate and psychological stress level in the case group were significantly higher than those in the control group(P<0.05).The psychological stress level of the male patients in the case group was significantly higher than that in the female group(P<0.05).With the increase of the patient′s age,the CS level,serum mast cell degranulation rate and psychological stress level all showed significant increases(P<0.05);the CS level of patients with platelet transfusion was positively correlated with the serum mast cell degranulation rate(r=0.363,P<0.05).The CS level was also positively correlated with psychological stress level(r=0.490,P<0.001).Serum mast cell degranulation rate was positively correlated with the psychological stress level(r=0.472,P<0.001).Conclusion There is a positive correlation between the level of CS,the degranulation rate of serum mast cells and the level of psychological stress in platelet transfusion patients.