采用基因芯片技术分析南通市抗酸杆菌的菌群分布特征及其耐药性研究

Analysis of Bacterial Distribution and Antimicrobial Resistance of Acid Fast Bacilli in Nantong City by Using Gene Chip Technology

目的探讨基因芯片技术分析本市抗酸杆菌的菌群分布特征及其耐药性的价值。方法选取我市2016年1月至2017年12月间收集的1436份抗酸杆菌阳性标本,其中1380份为痰液样本,38份为支气管肺泡灌洗液样本,18份为胸腹水样本;采用基因芯片技术分析抗酸杆菌的菌群分布,并分析各菌种的耐药性。结果痰液标本中MTBC占92. 75%,NTM占7.10%;胸腹水中MTBC占88.89%,NTM占11.11%;支气管肺泡灌洗液中MTBC占73.68%,NTM占21.05%。NTM对INH、RFP、EMB、SM、LFX及AMK耐药率均高于MTBC,且差异有统计学意义(P<0. 05); NTM的多重耐药、广泛耐药及总耐药率均显著高于MTBC(P<0.05)。结论采用基因芯片技术分析我市抗酸杆菌分布情况,MTBC检出率显著高于NTM;药敏试验结果显示,MTBC总耐药率为31.95%,NTM总耐药率为100%。

Objective To investigate the distribution characteristics and antimicrobial resistance of acid fast bacilli in Nantong city by using gene chip technology.Methods 1436 positive specimens of acid fast bacilli were collected from January 2016 to December 2017 in our city,of which 1380 were sputum samples,38 were bronchoalveolar lavage fluid samples and 18 were ascites and pleural fluid samples.Results MTBC accounted for 92.75%in sputum,7.10%in NTM,88.89%in pleural and ascitic fluid,11.11%in NTM,73.68%in bronchoalveolar lavage fluid and 21.05%in NTM.The resistance rates of NTM to INH,RFP,EMB,SM,LFX and AMK were significantly higher than those of MTBC,(P<0.05);The multidrug resistance,extensively drug resistance and total drug resistance rates of NTM were significantly higher than those of MTBC(P<0.05).Conclusion The distribution of acid-fast bacteria in our city was analyzed by using gene chip technology.The detection rate of MTBC is significantly higher than that of NTM;Drug sensitivity test show that the total drug resistance rate of MTBC is 31.95%,and the total resistance rate of NTM is 100%.