抗子宫内膜抗体和抗精子抗体应用于不孕者与习惯性流产者的检测价值研究

Study on the detection value of anti-endometrial antibody and antisperm antibody in infertility and habitual abortion

目的:探讨抗子宫内膜抗体与抗精子抗体应用于检测不孕者和习惯性流产者的临床价值。方法:选择2013年10月至2015年10月无锡市妇幼保健院收治的不孕者125例作为A组,选择同期收治的习惯性流产者125例作为B组,再选择同期进行常规检查的孕妇100例作为对照组。采用酶联免疫吸附法对三组人员抗子宫内膜抗体、抗精子抗体进行检测,并进行对比分析,总结抗子宫内膜抗体、抗精子抗体与不孕和习惯性流产的相关性。结果:A组患者抗子宫内膜抗体检测阳性率、抗精子抗体检测阳性率和总阳性率均高于对照组人员,其差异均具有统计学意义(均P <0. 05); B组患者抗子宫内膜抗体检测阳性率、抗精子抗体检测阳性率和总阳性率均高于对照组人员,其差异均具有统计学意义(均P <0. 05); B组患者孕周<12周者抗子宫内膜抗体检测阳性率、抗精子抗体检测阳性率和总阳性率均高于孕周≥12周患者,其差异均具有统计学意义(均P <0. 05)。结论:对抗子宫内膜抗体和抗精子抗体进行检测对诊断习惯性流产和不孕者具有重要临床价值,为不明原因的习惯性流产和不孕者提供了新的诊断依据,抗子宫内膜抗体和抗精子抗体与早期流产具有显著相关性。

Objective:To investigate the clinical value of anti-endometrial antibody and antisperm antibody in the detection of infertile and habitual abortion.Methods:From October 2013 to October 2015,125 cases of infertility admitted to Wuxi Maternal and Child Health Care Hospital were treated as group A,and 125 cases of habitual abortion were selected as group B in the same period.Then we selected routine examination in Wuxi Maternal and Child Health Care Hospital.100 cases of pregnant women as a control group.Three groups of anti-endometrial antibodies and anti-sperm antibodies were detected by enzyme-linked immunosorbent assay(ELISA),and the correlation between anti-sperm antibody and anti-sperm antibody and infertility and habitual abortion was summarized.Results:The positive rate of anti-endometrial antibody in group A was higher than that in control group,the difference was statistically significant(all P<0.05).The positive rate of anti-endometrial antibody in group B was higher than that in control group,the difference was statistically significant(all P<0.05).Group B gestational age<12 weeks endometrial antibody positive rate,the detection of anti-sperm antibodies positive rate and positive rate is higher than the total gestational age≥12 weeks,the difference was statistically significant(all P<0.05).Conclusions:Anti endometrial antibody and antisperm antibody detection in the diagnosis of habitual abortion and infertility has important clinical value,provides a new diagnostic method for unexplained habitual abortion and infertility,and anti endometrial antibody and anti sperm antibody and early abortion has a significant correlation.