叶黄素对H_2O_2作用下MIO-M1细胞活性及迁移的影响

Effect of lutein on viability and migration of MIO-M1 cells under H_2O_2-induced oxidative stress

目的:探讨不同浓度叶黄素对过氧化氢(H_2O_2)作用下人眼Müller细胞系MIO-M1细胞活性及迁移的影响。方法:将MIO-M1细胞随机分为对照组、H_2O_2损伤组及叶黄素+H_2O_2共处理组,采用四甲基偶氮唑盐比色法(MTT法)及改良细胞划痕法检测不同浓度H_2O_2及加入叶黄素共处理后MIO-M1细胞活性及迁移率。结果:MTT法显示,与对照组相比,H_2O_2损伤组MIO-M1细胞活性显著抑制;与H_2O_2损伤组相比,加入叶黄素共处理后可增加MIO-M1细胞活性,差异均有统计学意义(P <0.05)。改良细胞划痕法表明,与对照组相比,H_2O_2损伤组MIO-M1细胞迁移率显著下降;与H_2O_2损伤组相比,加入叶黄素共处理后可提高MIO-M1细胞迁移率,差异均有统计学意义(P <0.05)。结论:叶黄素对H_2O_2作用下MIO-M1细胞活性及迁移具有保护作用。

Objective:To investigate the effect of different concentrations of lutein on viability and migration of human Müller cell line MIO-M1cells under hydrogen peroxide(H2O2)induced oxidative stress.Methods:MIO-M1 cells were randomly divided into control group,H2O2 group and co-treatment(lutein+H2O2)group.The viability and migration of MIO-M1 cells which were treated with different concentrations of H2O2 and co-treatment with lutein were analyzed by MTT assay and modified wound scratch assay.Results:MTT assay showed that the viability of MIO-M1 cells was significantly decreased in the H2O2 group compared with the control group.The viability of MIO-M1 cells in co-treatment group were significantly increased compared with the H2O2 group(all P<0.05).Modified wound scratch assay indicated that the migration rate of MIO-M1 cells was significantly decreased in the H2O2 group compared with control group.The migration rate of MIO-M1 cells in co-treatment group was significantly increased compared with H2O2 group(all P<0.05).Conclusion:Lutein can protect the viability and migration of MIO-M1cells from H2O2-induced oxidative stress.