乙醇提取蛋黄粗磷脂的工艺研究

STUDY ON THE ETHANOL EXTRACTION OF CRUDE EGG YOLK PHOSPHOLIPIDS

以脱油蛋黄粉为原料,乙醇作为提取溶剂对脱油蛋黄粉中的磷脂进行提取。首先对提取溶剂乙醇体积分数进行选择,然后通过单因素试验,考察了提取时间、料液比、提取温度、搅拌速率和提取次数对乙醇提取蛋黄粗磷脂工艺效果的影响,以蛋黄粗磷脂的磷脂纯度和提取率为响应值,根据Box-Behnken中心组合试验设计原理对乙醇提取蛋黄粗磷脂的工艺条件进行了优化。结果表明:当乙醇体积分数为95%、提取时间为4 min、料液比为1∶5 g/mL、提取温度为65℃、搅拌速率为168 r/min、提取次数为2次时,蛋黄粗磷脂中的磷脂纯度和提取率均较高,磷脂纯度为71.03%,磷脂提取率为66.17%。

The phospholipids in deoiled egg yolk powder was extracted from deoiled egg yolk powder using ethanol as extraction solvent.First,the ethanol concentration was selected,and then the effects of extraction time,material-liquid ratio,extraction temperature,stirring rate,and extraction times on the effect of ethanol extraction of crude egg yolk phospholipids were investigated through single-factor experiments.Next,the response surface experiments were designed to optimize the reaction conditions of the ethanol extraction of crude egg yolk phospholipids according to the Box-Behnken center combination experimental design principle,and the purity and extraction rate of phospholipids in the crude egg yolk phospholipids were the response values.The optimal reaction conditions were as follows:ethanol concentration 95%,extraction time 4min,material-liquid ratio1∶5g/mL,extraction temperature 65℃,stirring rate 168r/min,and extraction twice.Based on the above parameters,the purity and extraction rate of phospholipids in the crude egg yolk phospholipids were71.03% and 66.17%.