管氏肿腿蜂寄生及注射其毒液对黄粉甲蛹营养代谢的影响

Effects of parasitization by Scleroderma guani and injection with its venom on nutritional metabolism of Tenebrio molitor pupae

本文以肿腿蜂科管氏肿腿蜂Scleroderma guani为研究对象,研究该蜂寄生及毒液对寄主黄粉甲Tenebrio molitor蛹营养代谢的影响。通过Braford法、香兰素-浓硫酸法、蒽酮法和3’5-二硝基水杨酸法分别测定管氏肿腿蜂寄生及注射其毒液对黄粉甲蛹血淋巴和脂肪体中蛋白质、脂类和糖类含量的影响。研究发现管氏肿腿蜂寄生和注射其毒液能引起黄粉甲蛹血淋巴中的蛋白质含量增加和脂类含量下降,而脂肪体中的蛋白质含量减少和脂类含量升高。同时,该蜂寄生和注射其毒液,均能引起黄粉甲蛹血淋巴和脂肪体中的总糖、海藻糖和还原糖含量升高,但糖原含量下降。研究结果表明,管氏肿腿蜂毒液能调控寄主黄粉甲蛹血淋巴和脂肪体中营养物质的代谢,为揭示该蜂对寄主的营养代谢调控机制奠定了基础。

In this study, effects of parasitization and venom of Scleroderma guani on nutritional metabolism of Tenebrio molitor pupae were investigated. After parasitization of S. guani or injection with its venom,protein,lipid and carbohydrate contents in hemolymph and fat body of T. molitor pupae were determined using the methods of Braford,vanillin-sulfuric acid,anthrone and 3'5-dinitro salicylic acid, respectively. The results revealed that protein and lipid contents in haemolymph of T. molitor pupae were respectively increased and decreased after parasitization and venom injection,while an opposite result was observed for fat body. In additon,parasitization and venom injection resulted in elevation of total carbohydrate,trehalose and reducing sugar,and reduction of glycogen. The results suggest that venom of S. guani can regulate the metabolism of nutrients in hemolymph and fat body of T. molitor,which lay a foundation for revealing the mechanisms of nutritional metabolism of the host regulated by S. guani.