通腑泄浊法对CKD模型大鼠肾功能及肠道屏障功能的影响

EFFECT OF THE METHOD "TONG FU XIE ZHUO" ON RENAL FUNCTION AND INTESTINAL BARRIER FUNCTION IN CKD MODEL RATS

目的探讨通腑泄浊法对慢性肾脏病(CKD)模型大鼠肠道屏障功能的影响,初步探讨其延缓CKD进展的机理。方法应用5/6肾切除法构建CKD大鼠模型,SD雄性大鼠120只随机分为空白组,模型组,通腑泄浊组(高、中、低剂量),药用炭组,每组20只,从模型建立后开始灌肠,疗程4周,第4周末检测血清肌酐,血硫酸吲哚酚水平,观察肾组织病理结构变化。结果 CKD模型组大鼠肾组织可见肾小管上皮细胞变性、间质炎性细胞浸润、纤维组织增生等病理变化,通腑泄浊组与CKD模型组大鼠相比较均有不同程度的改善,其中以通腑泄浊高剂量组改善最为明显。通腑泄浊各剂量组和药用炭组血内毒素、D-乳酸含量明显下降(P<0.05),且高剂量组比药用炭组下降更为明显(P<0.05)。结论通腑泄浊法具有一定的保护肾脏和抗肾间质纤维化的作用,降低大鼠血内毒素、D-乳酸含量,保护肠粘膜屏障功能可能是其机制之一。

Objective:To investigate the effect of the method of"Tong Fu Xie Zhuo"on the intestinal barrier function in rats with chronic kidney disease(CKD),and explore the mechanism of delaying the progress of CKD.Methods:The rat model of CKD was established by 5/6 nephrectomy.120 SD male rats were randomly divided into blank group,model group,high,middle and low dosage group(high,middle and low dosage)and medicinal charcoal group(20rats in each group).The rats were given with enema for4weeks.Serum creatinine was detected at the end of the 4th week.Results:Renal tubular epithelial cell degeneration was observed in CKD model group.The pathological changes,such as interstitial inflammatory cell infiltration,fibrous tissue proliferation and so on,were improved in different degrees in the group of purge turbidity of the Fu organs compared with the rats in the CKD model group,especially in the group of high dosage of purging turbidity of the Fu organs.The contents of endotoxin and D-lactic acid in blood of each dosage group and medical charcoal group were significantly decreased(P<0.05).The high dose group was significantly lower than that of the medicinal carbon group(P<0.05).Conclusion:Tthe method of"Tong Fu Xie Zhuo"can protect kidney and prevent renal interstitial fibrosis,and decrease the contents of endotoxin and D-lactic acid in blood of rats.Protection of intestinal mucosal barrier function may be one of its mechanisms.