ApoE基因敲除小鼠器官内特洛细胞的分布

Distribution of Telocytes in Vital Organs of ApoE^(-/-) Mice

目的发现和证实ApoE基因敲除小鼠重要器官内存在异质性间质细胞来源的特洛细胞,为进一步研究ApoE基因敲除小鼠疾病模型在相关疾病中发生发展的特异性改变提供实验基础证据。方法选取成年Apo E基因敲除小鼠动物模型作为研究对象,解剖切取心脏组织、肝脏组织和肾脏组织,通过选取不同免疫生物学指标行免疫组织化学染色。结果特洛细胞普遍存在于上述小鼠重要组织器官中,因器官不同而表现出不同的免疫组织化学生物标记蛋白,如心脏组织中特洛细胞对CD34、CD117和CD28免疫标记物阳性表达;肝脏组织中特洛细胞对血小板衍生生长因子受体-α免疫标记物阴性表达;肾脏组织中特洛细胞对CD117和血小板衍生生长因子受体-α免疫标记物阴性表达,而且特洛细胞在同一组织中的分布也存在差异。结论通过实验的方法得到了特洛细胞存在于ApoE^(-/-)小鼠体内重要器官的证据,并初步分析了特洛细胞免疫生物学指标的差异性表达。

Objective To identify and verify the distribution of Telocytes derived from heterogeneous interstitial cells in the vital organs of ApoE^-/-mice.Methods Heart,kidney,and liver tissues were harvested from ApoE^-/-adult mice.Immunohistochemical assays were performed by using different immunobiological markers.Results Telocytes were found in these vital organs.The expressions of immunobiological markers differed among different organs.CD34,CD117,and CD28 were positively expressed in Telocytes in cardiac tissue;CD117 and plateled-derived growth factor-αwere negatively expressed in Telocytes in renal tissue;and CD117 and plateled-derived growth factor receptor-αhad negative expression in Telocytes in hepatic tissue.Furthermore,the distribution of Telocytes also differed in the same organ.Conclusions Telocytes exist in the vital organs of ApoE^-/-mice,as demonstrated by immunohistochemisty assay.The expressions of immunobiological markers differ among Telocytes in different organs.