^(60)Co-γ射线诱变大豆“桂夏7号”突变体筛选

Screen of ^(60)Co-γ Ray Generated Mutants from Soybean 'Guixia7'

【目的】旨在研究玉米-大豆带状套作复合种植模式下大豆对荫蔽环境响应的分子机理,以期得到对荫蔽环境敏感程度较弱的大豆材料。【方法】以荫蔽敏感大豆品种"桂夏7号"为供试诱变亲本,利用^(60)Co-γ射线对其进行辐照处理,研究其对桂夏7号田间出苗率的影响并分析M1代农艺性状及品质变异。【结果】与对照相比,M1代田间出苗率仅为49.7%,^(60)Co-γ射线对种子萌发和出苗具有强烈抑制作用,M1代植株在株高、分枝数、蛋白质和油含量等方面与对照存在显著差异。其中,矮秆为0.77%,无分枝为0.56%,蛋白质含量和油含量与对照相比,分别高6.7%和5.4%,另筛选到7株粒形较大突变体。【结论】初步构建了含叶片、株型、生育期、粒形、籽粒蛋白质及油脂等突变类型的突变体库,突变植株数分别占总突变植株数的0.56%、3.58%、0.21%、0.98%、4.43%及0.49%,这些不同突变表型的突变体可为大豆的遗传学研究和育种提供有价值的材料。

【Objective】The aim of the study was to provide soybean materials with less sensitive to shade environment.【Method】The shade-sensitive soybean cultivar‘Guixia7’was irradiated with 60Co-γray in the present study,variations in field emergency rate of Guixia7 and the agronomic characters and seed quality of M1 were analyzed.【Result】The emergence rate of seeds in the field barely reached 49.7%in the M1 generation compared to the control,indicating that 60Co-γray significantly inhibits germination and emergence of soybean seeds.Mutants with different phenotypes of plant height,branch number,protein and oil contents were identified in M1 generation.In the mutant pools,the dwarf and non-branch phenotypes were 0.77%and 0.56%.In addition,one mutant has 6.7%and 5.4%higher protein and oil contents,respectively,than that in the control.Seven plants were found with larger seed size in the M1 mutant pools.【Conclusion】The soybean mutant pools consisting of mutants of leaf shape,plant architecture,growth period,grain shape,grain protein and oil contents have been preliminarily established.These mutants were accounting for 0.56%,3.58%,0.21%,0.98%,4.43%and 0.49%,respectively,which would provide valuable materials for soybean genetic research and breeding.